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3 results on '"Gregor Jung"'

1. A high-affinity fluorescence probe for copper(II) ions and its application in fluorescence lifetime correlation spectroscopy

Author

Thorsten Wohland, Andreas Grüter, Gregor Jung, Michael J. Hoffmann, Rolf Müller, and HIPS, Helmholtz-Institut für Pharmazeutische Forschung Saarland, Universitätscampus E8.1 66123 Saarbrücken, Germany.

Subjects
Boron Compounds, FLIM, Cations, Divalent, chemistry.chemical_element, Photochemistry, Biochemistry, Fluorescence, Analytical Chemistry, Ion, chemistry.chemical_compound, Transition metal, BODIPY, Humans, Molecule, Single molecule detection, Parkinson, Fluorescent Dyes, Tetrapeptide, Chemistry, ATCUN motif, Copper, Spectrometry, Fluorescence, Alzheimer, DAHK, Oligopeptides, Two-dimensional nuclear magnetic resonance spectroscopy
Abstract

Copper is one of the most important transition metals in many organisms where it catalyzes a manifold of different processes. As a result of copper's redox activity, organisms have to avoid unbound ions, and a dysfunctional copper homeostasis may lead to multifarious pathological processes in cells with very severe ramifications for the affected organisms. In many neurodegenerative diseases, however, the exact role of copper ions is still not completely clarified. In this work, a high-affinity and highly selective copper probe molecule, based on the naturally occurring tetrapeptide DAHK is synthesized. The sensor (log KD = - 12.8 ± 0.1) is tagged with a fluorescent BODIPY dye whose fluorescence lifetime distinctly decreases from 5.8 ns ± 0.2 ns to 0.4 ns ± 0.1 ns on binding to copper(II) cations. It is shown by using fluorescence lifetime correlation spectroscopy that the concentration of both probe and probe-copper complex can be simultaneously measured even at nanomolar concentration levels. This work presents a possible starting point for a new type of probe and method for future in vivo studies to further reveal the exact role of copper ions in organisms. Graphical abstract.

Published
2019

2. Fluorescent Proteins I : From Understanding to Design

Author

Gregor Jung and Gregor Jung

Subjects
Fluorescent polymers, Biofluorescence
Abstract

Fluorescent proteins are intimately connected to research in the life sciences. Tagging of gene products with fluorescent proteins has revolutionized all areas of biosciences, ranging from fundamental biochemistry to clinical oncology, to environmental research. The discovery of the Green Fluorescent Protein, its first, seminal application and the ingenious development of a broad palette of fluorescence proteins of other colours, was consequently recognised with the Nobel Prize for Chemistry in 2008. Fluorescent Proteins I is devoted to the basic photophysical and photochemical aspects of fluorescent protein technology. Experienced experts highlight colour tuning, the exploration of switching phenomena and respective methods for their investigation. The book provides a thorough understanding of primary molecular processes allowing the design of fluorescent proteins for specific applications.

Published
2012

3. Fluorescent Proteins II : Application of Fluorescent Protein Technology

Author

Gregor Jung and Gregor Jung

Subjects
Biofluorescence, Fluorescent polymers
Abstract

Fluorescent proteins are intimately connected to research in the life sciences. Tagging of gene products with fluorescent proteins has revolutionized all areas of biosciences, ranging from fundamental biochemistry to clinical oncology, to environmental research. The discovery of the Green Fluorescent Protein, its first, seminal application and the ingenious development of a broad palette of fluorescence proteins of other colours, was consequently recognised with the Nobel Prize for Chemistry in 2008. Fluorescent Proteins II highlights the physicochemical and biophysical aspects of fluorescent protein technology beyond imaging. It is tailored to meet the needs of physicists, chemists and biologists who are interested in the fundamental properties of fluorescent proteins, while also focussing on specific applications. The implementations described are cutting-edge studies and exemplify how the physical and chemical properties of fluorescent proteins can stimulate novel findings in life sciences.

Published
2012

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