1. Simultaneous three-dimensional imaging of multi-focal microscopy
- Author
-
Yuan Luo, Chen Yen Lin, and Yi-Tun Lin
- Subjects
Diffraction ,Physics ,Wavefront ,Polarized light microscopy ,Optics ,Multifocal plane microscopy ,business.industry ,Microscopy ,Phase (waves) ,Digital holographic microscopy ,Image plane ,business - Abstract
High-speed microscopy three-dimensional (3D) microscopy based on trans-illumination is implemented with an amplitude light modulator placed at the Fourier plane of the system. The phase of an incident wave-front is modified and encoded with a defocus parameter to divert the light onto different portion of an image plane depending on their diffraction order and depth positions. The design of the grating pattern for the light modulated is discussed through the simulation and the experiment. 3D imaging capability is demonstrated through the experiment.
- Published
- 2017