1. Biological pigments as fluorescent labels for cytometry
- Author
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Barbara B. Prézelin, Chia H. Chen, Diether J. Recktenwald, and John Kimura
- Subjects
Biological pigment ,medicine.diagnostic_test ,Chemistry ,Analytical chemistry ,Immunofluorescence ,Laser ,Fluorescence ,law.invention ,law ,Excited state ,medicine ,Biophysics ,Laser line ,Luminescence ,Cytometry - Abstract
The use of protein-based biological pigments in combination with low molecular weight organic dye molecules allows the simultaneous detection of up to six independent components of a cell surface by antibody dependent fluorescence (immunofluorescence) excited with a single 488nm laser line. This paper describes the state of the art in multi-color immunofluorescence with 488nm Ar-ion laser excitation. It provides the data to design systems for specialized applications.
- Published
- 1990
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