Aref Mostafazadeh, Yusuf Samet Yaras, Goksen G. Yaralioglu, Fehmi Civitci, Onur Cakmak, Hakan Urey, Ali Bars Gündüz, Gokhan Saglam, Ibrahim Baris, Selim Olcer, Özyeğin University, Yaralıoğlu, Göksenin, Yaras, Yusuf S., Gündüzö, Ali, Sağlam, Gökhan, Ölçer, Selim, Mustafazade, Ali, Barış, ibrahim (ORCID 0000-0003-2185-3259 & YÖK ID 111629), Ürey, Hakan, Cakmak, O., Civitci, F., Yaralioglu, G. G., College of Engineering, College of Sciences, Department of Electrical and Electronics Engineering, and Department of Department of Mathematics
We developed two types of cantilever-based biosensors for portable diagnostics applications. One sensor is based on MEMS cantilever chip mounted in a microfluidic channel and the other sensor is based on a movable optical fiber placed across a microfluidic channel. Both types of sensors were aimed at direct mechanical measurement of coagulation time in a disposable cartridge using plasma or whole blood samples. There are several similarities and also some important differences between the MEMS based and the optical fiber based solutions. The aim of this paper is to provide a comparison between the two solutions and the results. For both types of sensors, actuation of the cantilever or the moving fiber is achieved using an electro coil and the readout is optical. Since both the actuation and sensing are remote, no electrical connections are required for the cartridge. Therefore it is possible to build low cost disposable cartridges. The reader unit for the cartridge contains light sources, photodetectors, the electro coil, a heater, analog electronics, and a microprocessor. The reader unit has different optical interfaces for the cartridges that have MEMS cantilevers and moving fibers. MEMS based platform has better sensitivity but optomechanical alignment is a challenge and measurements with whole blood were not possible due to high scattering of light by the red blood cells. Fiber sensor based platform has relaxed optomechanical tolerances, ease of manufacturing, and it allows measurements in whole blood. Both sensors were tested using control plasma samples for activated-Partial-Thromboplastin-Time (aPTT) measurements. Control plasma test results matched with the manufacturer's datasheet. Optical fiber based system was tested for aPTT tests with human whole blood samples and the proposed platform provided repeatable test results making the system method of choice for portable diagnostics., Scientific and Technological Research Council of Turkey (TÜBİTAK)