1. FDISCO+: a clearing method for robust fluorescence preservation of cleared samples
- Author
-
Yusha Li, Tingting Yu, Xiaomei Liu, Jingtan Zhu, Peng Wan, Jianyi Xu, and Dan Zhu
- Subjects
Paper ,Fluorescence-lifetime imaging microscopy ,antioxidant ,Materials science ,Radiological and Ultrasound Technology ,Neuroscience (miscellaneous) ,Research Papers ,Fluorescence ,fluorescence preservation ,fluorescence imaging ,Optical clearing ,FDISCO+ ,optical clearing ,Clearing ,Radiology, Nuclear Medicine and imaging ,Clearing Agent ,room temperature ,Biological system ,Imaging condition ,Clearance - Abstract
Significance: The recently reported solvent-based optical clearing method FDISCO can preserve various fluorescent signals very well. However, the strict low-temperature storage condition of FDISCO is not conducive to long-time or repetitive imaging usually conducted at room temperature. Therefore, it is important to solve the contradiction between fluorescence preservation and imaging condition. Aim: We develop a modified FDISCO clearing method, termed FDISCO+, to change the preservation condition from low temperature to room temperature. Approach: Two alternative antioxidants were screened out to effectively inhibit the peroxide generation in the clearing agent at room temperature, enabling robust fluorescence preservation of cleared samples. Results: FDISCO+ achieves comparable fluorescence preservation with the original FDISCO protocol and allows long-time storage at room temperature, making it easier for researchers to image and preserve the samples. Conclusions: FDISCO+ is expected to be widely used due to its loose operation requirements.
- Published
- 2021