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Daily food habit with anti-allergic activities is expected to prevent the onset of allergic diseases and ameliorate allergic symptoms. The red color of ripe tomato fruit is due mainly to the accumulation of the carotenoid all-trans-lycopene, which is produced during fruit ripening. Red tomato peel extract (RTPE) contain lycopene (450mg/ kg) and has been found to have anti-allergic effects on man compare its flesh extract and yellow tomato peel extract (YTPE). RTPE could possibly inhibit histamine release and relieve the symptoms of all types of allergy including cedar pollinosis. To evaluate the anti-allergic effect of RTPE, we performed a research study randomized, on three groups in 50 perennial allergy patients with red peel extract; yellow peel extract; tomato flesh and Dextrin (20 healthy university students as control). All patients using oral administration of red tomato peel extract (RTPE); yellow/pink tomato peel extract (YTPE) and tomato flesh extract (TFE) 30mg per day /patient each group and Dextrin as control (30mg per day /patient each group) for 56 days. We found that the skin test for allergy score significantly decreased in the RTPE group only at the end of the trial compared to the beginning and treated with YTPE and TFE groups. Besides this, we also found that sneezing score decreases significantly at the end of experiment time compare to the beginning (p

Background: Allergy to pollen from gymnosperms is well documented in the west. The objective was to define the allergologic protein from Litchi chinensis (Litchi) pollen and conjugate the protein with polysaccharides by Maillard reaction to reduce the allergic effect of that protein. Methods: Total soluble proteins were extracted from the pollen of Litchi flower pollen and subjected to ammonium sulphate precipitation at 80% saturation. Pollen antigen from Litchi chinensis (Litchi) was prepared by gel cutting method and characterized by biochemical and designated by LFPP. The homogeneity of this protein was demonstrated by a single band on SDS-PAGE. The protein then conjugated with galactomannan through Maillard Reaction. The resulting purified pollen protein and conjugated protein were administered to the Swiss albino mice as amount of 5.8mg/kg body weight. Results: The total protein was then separated on a 12% SDS-Polyacrylamide gel which revealed 5 bands between molecular weight range of 29kDa and 69kDa. Each band was recovered from the gel by electroelution and sent for skin tests. 28kDa proteins was the only allergenic protein while others were not shown reactivity in patients. Intraperitoneal injection of the purified protein (LFPP) caused a significant rise in the levels of neutrophils (38-81%) and eosinophils (3-14%) compared to control (P