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Your search keyword '"Miyamae, Takeaki"' showing total 5 results
Start Over Author "Miyamae, Takeaki" Publisher society for neuroscience
5 results on '"Miyamae, Takeaki"'

4. A seven-transmembrane receptor that mediates avoidance response to dihydrocaffeic acid, a water-soluble repellent in Caenorhabditis elegans

Author

Aoki, Reina, Yagami, Tatsorou, Sasakura, Hiroyiki, Ogura, Ken-ichi, Kajihara, Yasuhiro, Ibi, Masakazu, Miyamae, Takeaki, Nakamura, Fumio, Asakura, Taro, Kanai, Yoshikatsu, Misu, Yoshimi, Iino, Yuichi, Ezcurra, Marina, Schafer, W., Mori, Ikue, Goshima, Yoshio, Aoki, Reina, Yagami, Tatsorou, Sasakura, Hiroyiki, Ogura, Ken-ichi, Kajihara, Yasuhiro, Ibi, Masakazu, Miyamae, Takeaki, Nakamura, Fumio, Asakura, Taro, Kanai, Yoshikatsu, Misu, Yoshimi, Iino, Yuichi, Ezcurra, Marina, Schafer, W., Mori, Ikue, and Goshima, Yoshio

Abstract

The ability to detect harmful chemicals rapidly is essential for the survival of all animals. In Caenorhabditis elegans (C. elegans), repellents trigger an avoidance response, causing animals to move away from repellents. Dihydrocaffeic acid (DHCA) is a water-soluble repellent and nonflavonoid catecholic compound that can be found in plant products. Using a Xenopus laevis (X. laevis) oocyte expression system, we identified a candidate dihydrocaffeic acid receptor (DCAR), DCAR-1. DCAR-1 is a novel seven-transmembrane protein that is expressed in the ASH avoidance sensory neurons of C. elegans. dcar-1 mutant animals are defective in avoidance response to DHCA, and cell-specific expression of dcar-1 in the ASH neurons of dcar-1 mutant animals rescued the defect in avoidance response to DHCA. Our findings identify DCAR-1 as the first seven-transmembrane receptor required for avoidance of a water-soluble repellent, DHCA, in C. elegans.

Published
2011

5. A seven-transmembrane receptor that mediates avoidance response to dihydrocaffeic acid, a water-soluble repellent in Caenorhabditis elegans.

Author

Aoki R, Yagami T, Sasakura H, Ogura K, Kajihara Y, Ibi M, Miyamae T, Nakamura F, Asakura T, Kanai Y, Misu Y, Iino Y, Ezcurra M, Schafer WR, Mori I, and Goshima Y

Subjects
3,4-Dihydroxyphenylacetic Acid pharmacology, Analysis of Variance, Animals, Animals, Genetically Modified, Behavior, Animal drug effects, Caenorhabditis elegans physiology, Caenorhabditis elegans Proteins chemistry, Caenorhabditis elegans Proteins genetics, Catechols pharmacology, Cloning, Molecular methods, Dose-Response Relationship, Drug, Escape Reaction physiology, Hydroxybenzoates, Larva, Luminescent Proteins genetics, Luminescent Proteins metabolism, Membrane Potentials drug effects, Membrane Potentials genetics, Microinjections methods, Models, Molecular, Mutation genetics, Receptors, G-Protein-Coupled genetics, Sensory Receptor Cells drug effects, Sensory Receptor Cells physiology, Xenopus, Caenorhabditis elegans Proteins metabolism, Caffeic Acids pharmacology, Escape Reaction drug effects, Receptors, G-Protein-Coupled metabolism
Abstract

The ability to detect harmful chemicals rapidly is essential for the survival of all animals. In Caenorhabditis elegans (C. elegans), repellents trigger an avoidance response, causing animals to move away from repellents. Dihydrocaffeic acid (DHCA) is a water-soluble repellent and nonflavonoid catecholic compound that can be found in plant products. Using a Xenopus laevis (X. laevis) oocyte expression system, we identified a candidate dihydrocaffeic acid receptor (DCAR), DCAR-1. DCAR-1 is a novel seven-transmembrane protein that is expressed in the ASH avoidance sensory neurons of C. elegans. dcar-1 mutant animals are defective in avoidance response to DHCA, and cell-specific expression of dcar-1 in the ASH neurons of dcar-1 mutant animals rescued the defect in avoidance response to DHCA. Our findings identify DCAR-1 as the first seven-transmembrane receptor required for avoidance of a water-soluble repellent, DHCA, in C. elegans.

Published
2011
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