1. Viral etiology of acute respiratory infections in children in Southern Iran
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Mehrdad Halaji, Tayebeh Hashempour, Javad Moayedi, Gholam Reza Pouladfar, Behzad Khansarinejad, Reza Khashei, Afagh Moattari, Zahra Musavi, Farzaneh Ghassabi, and Neda Pirbonyeh
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Acute respiratory infections ,Respiratory syncytial vírus ,Influenza A vírus ,Human metapneumovirus ,Real-time polymerase chain reaction ,Arctic medicine. Tropical medicine ,RC955-962 - Abstract
Abstract INTRODUCTION: Prevalence of influenza A virus (Flu-A), respiratory syncytial virus (RSV), and human metapneumovirus (hMPV) was assessed in children with acute respiratory infections (ARIs). METHODS: Nasopharyngeal aspirates and throat swabs were subjected to real-time polymerase chain reaction (PCR) to detect RSV and Flu-A and to conventional PCR to detect hMPV. RESULTS: Of the 156 children assessed, 93 (59.6%) carried at least one virus, with 35.9% positive for RSV, 14.1% for hMPV, and 9.6% for Flu-A. The prevalence of co-infections was 2.6%. CONCLUSIONS: The high detection rate may reflect increased sensitivity of real-time PCR compared to traditional PCR and viral culture.
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