1. Comparative cytogenetic survey of the giant bonytongue Arapaima fish (Osteoglossiformes: Arapaimidae), across different Amazonian and Tocantins/Araguaia River basins
- Author
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Terumi Hatanaka, Manolo F. Perez, Eliana Feldberg, Patrik F. Viana, Marcelo de Bello Cioffi, Ezequiel Aguiar de Oliveira, Luiz Antonio Carlos Bertollo, Edivaldo Herculano Corrêa de Oliveira, Francisco de Menezes Cavalcante Sassi, Petr Ráb, and Tariq Ezaz
- Subjects
0106 biological sciences ,Bacia do Rio Tocantins-Araguaia ,Amazonian ,Drainage basin ,Chromosome banding ,DNAr FISH ,Aquatic Science ,Osteoglossiformes ,010603 evolutionary biology ,01 natural sciences ,An?lise Citogen?tica / veterin?ria ,03 medical and health sciences ,Pirarucu ,Arapaima ,Peixes / anatomia & histologia ,Bandamento cromossômico ,Osteoglossomorpha ,Fish cytotaxonomy ,rDNA FISH ,Ecology, Evolution, Behavior and Systematics ,Bacia do Rio Amazonas ,030304 developmental biology ,CGH ,Arapaimidae ,0303 health sciences ,geography ,geography.geographical_feature_category ,biology ,biology.organism_classification ,Fishery ,QL1-991 ,%22">Fish ,Animal Science and Zoology ,Mapeamento Cromoss?mico ,Zoology ,Citotaxonomia ,DNA Riboss?mico - Abstract
Conselho Nacional de Desenvolvimento Cient?fico e Tecnol?gico (CNPq) (401962/2016?4 and 302449/2018?3 to MBC); Funda??o de Amparo ? Pesquisa do Estado de S?o Paulo (FAPESP) (2018/22033?1 to MBC; and 2017/10240?0 to MFP); Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior (CAPES)/Alexander von Humboldt Foundation (88881.136128/2017?01 to MBC). project EXCELLENCE (CZ.02.1.01/0.0/0.0/ 15_003/0000460 OP). Universidade Federal de S?o Carlos. Departamento de Gen?tica e Evolu??o. S?o Carlos, SP, Brazil / Secretaria de Estado de Educa??o de Mato Grosso. Cuiab?, MT, Brazil. Universidade Federal de S?o Carlos. Departamento de Gen?tica e Evolu??o. S?o Carlos, SP, Brazil. Universidade Federal de S?o Carlos. Departamento de Gen?tica e Evolu??o. S?o Carlos, SP, Brazil. Universidade Federal de S?o Carlos. Departamento de Gen?tica e Evolu??o. S?o Carlos, SP, Brazil. Czech Academy of Sciences. Institute of Animal Physiology and Genetics. Laboratory of Fish Genetics. Czech Republic. University of Canberra. Instituto for Applied Ecology. Canberra, ACT, Australia. Universidade Federal de S?o Carlos. Departamento de Gen?tica e Evolu??o. S?o Carlos, SP, Brazil. Instituto Nacional de Pesquisas da Amaz?nia. Coordena??o de Biodiversidade. Laborat?rio de Gen?tica Animal. Petr?polis, AM, Brazil. Instituto Nacional de Pesquisas da Amaz?nia. Coordena??o de Biodiversidade. Laborat?rio de Gen?tica Animal. Petr?polis, AM, Brazil. Minist?rio da Sa?de. Secretaria de Vigil?ncia em Sa?de. Instituto Evandro Chagas. Laborat?rio de Cultura de Tecidos e Citogen?tica. Ananindeua, PA, Brasil. Universidade Federal de S?o Carlos. Departamento de Gen?tica e Evolu??o. S?o Carlos, SP, Brazil. The South American giant fishes of the genus Arapaima, commonly known as pirarucu, are one of the most iconic among Osteoglossiformes. Previously cytogenetic studies have identified their karyotype characteristics; however, characterization of cytotaxonomic differentiation across their distribution range remains unknown. In this study, we compared chromosomal characteristics using conventional and molecular cytogenetic protocols in pirarucu populations from the Amazon and Tocantins-Araguaia river basins to verify if there is differentiation among representatives of this genus. Our data revealed that individuals from all populations present the same diploid chromosome number 2n=56 and karyotype composed of 14 pairs of meta-to submetacentric and 14 pairs of subtelo-to acrocentric chromosomes. The minor and major rDNA sites are in separate chromosomal pairs, in which major rDNA sites corresponds to large heterochromatic blocks. Comparative genomic hybridizations (CGH) showed that the genome of these populations shared a great portion of repetitive elements, due to a lack of substantial specific signals. Our comparative cytogenetic data analysis of pirarucu suggested that, although significant genetic differences occur among populations, their general karyotype patterns remain conserved.
- Published
- 2020