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To evaluate the potential of seed priming tools in soybean salt stress tolerance with improved plant growth and biomass content. Soybean seeds were primed with 5 different priming agents (proline, sodium nitroprusside (SNP), glycienbetaine, hydrogen peroxide & mannitol) then germination under laboratory conditions using 100mM NaCl stress condition was evaluated. Results indicated that SNP (nitric oxide donor) was found most effective agent for growth promotion, while unprimed treatment decreased germination, growth and biomass related parameters. SNP-primed seeds had a higher germination percentage (82%) and seedlings were exhibited increased proline content (105%) as compared to unprimed treatment. The protective mechanism of SNP against oxidative stress was correlated with lower lipid peroxidation (MDA content). Notably, the ability to maintain biomass level (41% reduction) as well as chlorophyll content indicated a role of SNP in alleviation of salt stress and induce tolerance. Altogether, our results highlight that exogenously SNP could be employed to attain better growth and development of soybean and perhaps other legumes under salt stress.

The present study was designed to elevate the in vitro bacterial mechanisms related to the plant growth promotion and their tolerance for sodium chloride (NaCl) and polyethylene glycol (PEG) in culture media. Total nine bacterial strains were studied for both stress tolerance under varying concentration of NaCl and PEG. Out of them, three bacterial strains namely Pseudomonas simiae AU, P. koreensis AK-1 and Carnobacterium sp. SJ-5 were found tolerate to stress and further used for biochemical characterization of ACC-deaminase, IAA and Pi-solubilization activities under both stresses. All three strains were exhibited equal amount of Pisolubilization at each stress levels. The strain P. simiae AU significantly presented the highest ACC-deaminase activity (81 nmol/mg/h and 73 nmol/mg/h) and IAA activity (41.5 Hg/mL and 39.08 Hg/mL) at 0.4M NaCl and 10% PEG stress respectively.

The present study was designed to elevate the in vitro bacterial mechanisms related to the plant growth promotion and their tolerance for sodium chloride (NaCl) and polyethylene glycol (PEG) in culture media. Total nine bacterial strains were studied for both stress tolerance under varying concentration of NaCl and PEG. Out of them, three bacterial strains namely Pseudomonas simiae AU, P. koreensis AK-1 and Carnobacterium sp. SJ-5 were found tolerate to stress and further used for biochemical characterization of ACC-deaminase, IAA and Pi-solubilization activities under both stresses. All three strains were exhibited equal amount of Pisolubilization at each stress levels. The strain P. simiae AU significantly presented the highest ACC-deaminase activity (81 nmol/mg/h and 73 nmol/mg/h) and IAA activity (41.5 Hg/mL and 39.08 Hg/mL) at 0.4M NaCl and 10% PEG stress respectively.

To evaluate the potential of seed priming tools in soybean salt stress tolerance with improved plant growth and biomass content. Soybean seeds were primed with 5 different priming agents (proline, sodium nitroprusside (SNP), glycienbetaine, hydrogen peroxide & mannitol) then germination under laboratory conditions using 100mM NaCl stress condition was evaluated. Results indicated that SNP (nitric oxide donor) was found most effective agent for growth promotion, while unprimed treatment decreased germination, growth and biomass related parameters. SNP-primed seeds had a higher germination percentage (82%) and seedlings were exhibited increased proline content (105%) as compared to unprimed treatment. The protective mechanism of SNP against oxidative stress was correlated with lower lipid peroxidation (MDA content). Notably, the ability to maintain biomass level (41% reduction) as well as chlorophyll content indicated a role of SNP in alleviation of salt stress and induce tolerance. Altogether, our results highlight that exogenously SNP could be employed to attain better growth and development of soybean and perhaps other legumes under salt stress.