1. The Cell Survival of F10B16 Melanoma and 4T1 Breast Adenocarcinoma Irradiated to Gamma Radiation Using the MTT Assay Based on Two Different Calculation Methods
- Author
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Nikzad, Safoora, Hashemi, Bijan, Hassan, Zuhair-Mohammad, and Mozdarani, Hossein
- Subjects
MTT assay ,4T1 breast adenocarcinoma ,Original Article ,F10B16 melanoma ,ionizing radiation ,cell survival fraction ,growing curve - Abstract
Background: various MTT assay methods are proposed to obtain the cell survival parameters. Objective: determining the survival curve characteristics of two cancerous cells of interest based on a common and a novel MTT assay method after exposing them to ionizing radiation. Methods: a common and a novel MTT assay method were used and compared for obtaining the F10B16 melanoma and 4T1 breast adenocarcinoma survivals after exposing them to ionizing radiation from a Co-60 machine. To obtain the survival parameters of the cells based on the common method, the cells were inoculated in 96-well plates. After irradiating the plates, the MTT assay was performed over the following days for a period of 8 days. Thereafter, the survival fraction was calculated from a simple equation for every day from which the best day was selected. To acquire the cells’ survival parameters based on the novel method, extensive experiments were performed on a large number of samples. Then, the MTT assay was done in every day following various experimental treatments to acquire the exponential growth. Finally, the cells’ survivals were determined by measuring the space between relevant growing curves. Results: at low doses (
- Published
- 2013