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目的　观察高原旅居史对急进高原人群适应能力的影响。方法　选择2023年11月—2024年1月急进高 原地区（3 900 m）的青年士兵2 337人，按照高原旅居史次数分为无高原旅居史组（A组，425人）、2 ~ 3次高原旅居史 组（B组，1 534人）、4次及以上高原旅居史组（C组，378人）。三组乘坐汽车由驻地出发上升至海拔3 900 m地区，用 时9 h，中途休整约1 h。分别于三组进入高原前（第0天）及急进高原后第1、7、14天进行急性高原病（AMS）评分，检 测血氧饱和度、心率、红细胞（RBC）、血红蛋白（HGB）、红细胞压积（Hct）、体质量、体脂率，于急进高原第14天进行 匹兹堡睡眠质量指数量表（PSQI）评分。结果　A组急进高原第1、7天AMS评分高于B、C组（P 均<0. 01）。A、B、C 组急进高原第14天AMS发生率依次降低（P 均<0. 01）。A、B、C组急进高原第1、7天血氧饱和度低于第0天，第1、7、 14天心率高于第0天，第7、14天RBC、HGB、Hct高于第0天（P 均<0. 05）；急进高原第1、7天，B、C组血氧饱和度高于 A组，第1天C组血氧饱和度高于B组（P 均<0. 05）；急进高原第7、14天A、B、C组的RBC、HGB、Hct依次增高（P 均 <0. 05）。三组急进高原后体质量和体脂率变化差异均无统计学意义（P 均>0. 05）。急进高原第14天A、B、C组PSQI 总分依次降低，其中日间功能、睡眠效率、睡眠时间、入睡时间、睡眠质量项目得分也依次降低（P 均<0. 05）。结论 多次高原旅居史者较无高原旅居史者急进高原后AMS及睡眠障碍发生率降低，有高原旅居史人群对高原的适应能 力更强。 [ABSTRACT FROM AUTHOR]

目的分析学龄期(6~12岁)儿童功能性便秘发生的影响因素,为防治学龄期儿童功能性便秘提供参 考。方法纳入1300例西宁市学龄期儿童,采用自制学龄期儿童功能性便秘调查问卷表分析功能性便秘发生情 况,采用单因素及多因素 Logistic回归分析法分析西宁市学龄期儿童功能性便秘发生的影响因素。结果1253例 学龄期儿童中功能性便秘者132例,学龄期儿童FC患病率10.5%(132/1253)。功能性便秘儿童与健康儿童在家族 便秘史、居住面积、过敏、孩子出生时分娩方式、母乳喂养持续时间、是否规律食用早餐、每日饮水量、蔬菜水果食摄 入占比、吃饭方式、挑食、是否喜食辛辣刺激性食物及进食粗粮频率、每周的运动时间、是否接受过如厕训练、每天排 便习惯的养成、每天睡眠时间、性格特点等指标间比较,P均<0.05。家族便秘史、母乳喂养持续时间、每日饮水量、 挑食、过敏、是否喜食辛辣刺激性食物摄入、孩子每周运动量、是否接受过如厕训练、是否养成每天排便习惯、进食粗 粮频率是学龄期儿童功能性便秘发生的影响因素(OR分别为1.804、0.755、1.393、0.651、0.719、0.569、0.906、 1. 138、1.813、1.868、1.758、0.668、0.498、0.559、0.971、0.715、0.625,P均<0.05)。结论学龄期儿童功能性便秘 的发生率较高。学龄期儿童发生功能性便秘发生的影响因素包括家族便秘史、母乳喂养持续时间及挑食等。 [ABSTRACT FROM AUTHOR]

慢性阻塞性肺疾病(COPD)是以不可逆气流受限为特征的呼吸系统慢性气道炎症性疾病, 发掘可靠的生 物标志物是 COPD 早期诊断并及时干预的重要手段. 目前 COPD 的诊断主要依赖于病史采集和肺功能检测, 临床 上尚缺乏敏感度和特异度较高的早期生物标志物以辅助诊断. 组学技术是-种将高通量技术应用于生命科学研究 的方法, 包括代谢组学、蛋白质组学、转录组学等多个方面的技术, 可对生物体各阶段的代谢产物、蛋白质和基因表 达情况进行研究. 近年来, 组学技术的发展和应用为COPD的诊断带来了新思路, 通过组学技术对COPD发生发展 动态变化过程的探索, 可为COPD的诊断及鉴别提供支持. [ABSTRACT FROM AUTHOR]

Copyright of Shandong Medical Journal is the property of Shandong Medical Health Newspapers and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Shandong Medical Journal is the property of Shandong Medical Health Newspapers and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Shandong Medical Journal is the property of Shandong Medical Health Newspapers and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Shandong Medical Journal is the property of Shandong Medical Health Newspapers and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Shandong Medical Journal is the property of Shandong Medical Health Newspapers and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

目的 分析肺内外多发结核分枝杆菌（MTB）感染患者的发病模式。方法 选择肺内外多发MTB感染患 者153例，通过痰标本、胸腔积液标本等共分离391株MTB菌株，通过BACTEC MGIT 960液体药敏实验分析菌株异 质性耐药情况，通过 MIRU-VNTR、全基因组测序方法，对 MTB菌株进行基因分型，判定患者发病模式。结果 153 例患者的发病模式为内源性复燃或迁移感染146例，混合感染5例，外源性再感染1例，菌株微进化合并外源性再感 染 1 例。3 例发病模式为混合感染的患者存在 MTB 菌株异质性耐药。发病部位均为肺部与泌尿系统。3 例患者 MTB菌株表型耐药结果与耐药基因位点突变结果一致。结论 肺内外多发MTB感染患者的发病模式以内源性复 燃或迁移感染为主，且MTB菌株可出现异质性耐药。 [ABSTRACT FROM AUTHOR]

Copyright of Shandong Medical Journal is the property of Shandong Medical Health Newspapers and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Shandong Medical Journal is the property of Shandong Medical Health Newspapers and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Shandong Medical Journal is the property of Shandong Medical Health Newspapers and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Shandong Medical Journal is the property of Shandong Medical Health Newspapers and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

目的观察超声分散技术前处理的痰标本的分枝杆菌培养效果。方法 387例临床疑似结核病的患者痰液标本400例份,分为实验组及对照组,实验组采用超声分散技术进分支杆菌培养前处理,在超声分散1 min后静置5min。对照组采用简单法前处理方法。前处理后取实验组及对照组进行分枝杆菌培养,观察并记录各组出现菌株生长并判定为抗酸杆菌阳性的时间时间,测算各组分枝杆菌培养阳性率、标本污染率。结果实验组及对照组出现阳性菌株时间分别为(20±8. 11)、(20±7. 67) d。实验组、对照组分枝杆菌培养阳性率分别为30. 0%(120/400)、23. 5%(94/400)(P <0. 05)。实验组、对照组培养污染率分别为1. 0%(4/400)、4. 8%(19/400)(P <0. 05)。对照组生长并判为细菌污染而被实验组报告阳性的为13例份,对照组因菌株不生长判为阴性而实验组最后报告阳性的为13例份。结论超声分散技术前处理的痰标本分枝杆菌培养阳性检出率高,污染率低,且所需时间短。 [ABSTRACT FROM AUTHOR]

Copyright of Shandong Medical Journal is the property of Shandong Medical Health Newspapers and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Shandong Medical Journal is the property of Shandong Medical Health Newspapers and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Shandong Medical Journal is the property of Shandong Medical Health Newspapers and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

目的探讨术前血清白蛋白水平检测对结直肠癌患者根治术后预后的评估价值。方法选择行根治术 并获得长期随访的187例结直肠癌患者。术前检测患者血清白蛋白水平，将术前血清白蛋白矣37.0 g/L的患者92 例作为水平降低组，>37.0 g/L的患者95例作为水平正常组。对比分析两组临床资料。术后随访5年，统计患者 生存情况及复发情况。采用Kaplan-Meier进行生存分析，采用COX回归进行多因素分析。结果两组性别、年 龄、肿瘤部位比较，P均>0. 05;水平降低组肿瘤直径≥5 cm、低分化、浸润深度T4期、有淋巴结转移的比例高于水 平正常组(P均<0.05)。水平降低组中位生存时间低于水平正常组，复发率高于水平正常组（P均<0.05)。浸润 深度、淋巴结转移及血清白蛋白水平是根治术后结直肠癌患者预后的影响因素（P均<0.05)。结论术前血清白 蛋白水平检测可评估结直肠癌患者根治术后的预后;血清白蛋白水平越低患者预后越差。 [ABSTRACT FROM AUTHOR]

Copyright of Shandong Medical Journal is the property of Shandong Medical Health Newspapers and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

目的：转染Smad3-siRNA的活化肝星状细胞（HSCs） Smad3表达及细胞外基质分泌量变化。方法利用生物软件进行Smad3 mRNA模拟，选择合适的靶位点，使用pSilencer3．1-H1-hygro载体表达Smad3特异性siRNA。将Smad3-siRNA真核表达载体转染HSC-T6细胞株，将HSC-T6细胞接种到24孔培养板，待细胞生长融合达60％～80％后，每3孔为1组，共分为4组，HSC-T6组细胞未经过质粒转染；空白质粒组细胞经过不含Smad3-siRNA的空白质粒转染；1μg Smad3-siRNA转染组细胞经过含有1μg Smad3-siRNA的质粒转染；2μg Smad3-siRNA转染组细胞经过含有2μg Smad3-siRNA的质粒转染。应用RT-PCR法、Western blotting法分别检测Smad3 mRNA和蛋白，同时检测细胞上清胶原Ⅲ。结果成功构建siRNA表达克隆。 HSC-T6组、空白质粒组、1μg Smad3-siRNA组、2μg Smad3-siRNA组的Smad3 mRNA相对表达量分别为0．98±0．06、0．98±0．10、0．72±0．10、0．31±0．15，Smad3蛋白相对表达量分别为0．98±0．01、0．96±0．02、0．73±0．03、0．30±0．02，胶原Ⅲ含量分别为33．11±0．45、33．55±1．00、17．93±1．05、11．80±1．31，1μg Smad3-siRNA组、2μg Smad3-siRNA组分别与HSC-T6组比较，1μg Smad3-siRNA组、2μg Smad3-siRNA组分别与空白质粒组比较，1μg Smad3-siRNA组与2μg Smad3-siRNA组比较，P均＜0．05。结论在体外实验中真核表达载体Smad3-siRNA可有效地抑制HSCs中Smad3的表达，并可减少激活的HSCs分泌细胞外基质。 [ABSTRACT FROM AUTHOR]

Objective To investigate the value of plasma total homocysteine (tHcy) in diagnosis of lacunar infarction, and the relationship with cerebrovascular hemodynamic accumulative score (CVHS).Methods Ninety cases of pa-tients with acute lacunar infarction were selected .According to the tHcy levels, the patients were divided into high tHcy group (tHcy≥10.0 μmol/L, 66 cases) and normal tHcy group (tHcy <10.0 μmol/L, 24 cases). The cerebrovascular hemodynamic parameters ( CVHP) of bilateral carotid artery were measured by using cerebral blood vessel analyzer, and CVHS was calculated. The correlation between tHcy and CVHS was analyzed. Results The values of Vmax, Vmin, Vmean, Qmean and CVHS in the high tHcy group were lower than those of the normal tHcy group , and the peripheral resistance (Rv), dynamic resistance (DR), critical pressure (CP), pulse wave velocity (Wv), characteristic impedance (Zcv), divergence rate (DI) and differential pressure (DP) were higher than those of the normal tHcy group (all P<0.05). The plasma tHcy level was negatively correlated with CVHS in the high tHcy group (r=-0.440, P<0.05), and no significant correlation between them was found in the normal tHcy group .Conclusion The plasma tHcy level was negatively correlated with CVHS in patients with high tHcy , and the elevated plasma tHcy levels may be related with the cerebral vascular dysfunction in patients with lacunar infarction. [ABSTRACT FROM AUTHOR]

目的探讨血清清道夫受体(CD36)及单核细胞趋化蛋白1 (MCP-1)水平变化与颈动脉粥样硬化斑块的 关系。方法选取颈动脉粥样硬化斑块患者50例(颈动脉斑块组)及健康体检者50例(颈动脉正常组)，比较两 组及斑块不同分级组患者血清中CD36、MCP-1水平，分析其与颈动脉粥样硬化斑块的相关性。结果与颈动脉正 常组相比，颈动脉斑块组患者血清中CD36、MCP-1水平均明显升髙(P均<0.05)，且随着斑块临床分级的增髙， CD36、MCP-1水平均逐渐升髙(P均<0.05)。相关性分析显示血清CD36及MCP-1水平与颈动脉粥样硬化斑块分 级均呈正相关(r分别为0. 869、0. 904,P均<0.05)。结论血清CD36及MCP-1水平与颈动脉粥样硬化斑块密切 相关，且随着斑块临床分级的增高而水平逐渐升高。 [ABSTRACT FROM AUTHOR]

目的　观察志贺菌临床耐药情况及非典型1类整合子基因盒的携带情况。方法　采用K-B纸片法对天津地区3所三级甲等医院分离的159株志贺菌进行药敏试验,分析其耐药情况。采用RT-PCR法检测志贺菌1类整合子基因盒携带情况,并进行基因测序分析其耐药基因盒类型。结果 159株志贺菌中,对3种及3种以上抗菌药耐药148株(93.1%),抗生素耐药情况分别为四环素85.5%、复方新诺明82.4%、氨苄西林82.4%;携带1类整合子基因盒134株,其中非典型1类整合子106株(66.7%);非典型1类整合子可变区携带的耐药基因盒blaox a-30-aad A1(6.6%)、aad A5R(4.7%)、dfr A12R(57.5%)。结论　志贺菌多药耐药现象较明显,菌株携带非典型1类整合子比例较高,其主要可变区耐药基因盒种类为dfr A12R。 [ABSTRACT FROM AUTHOR]

Copyright of Shandong Medical Journal is the property of Shandong Medical Health Newspapers and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)