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Copyright of Shandong Medical Journal is the property of Shandong Medical Health Newspapers and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Objective To explore the effects of intraperitoneal injection of interleukin-37（IL-37）on myocardial in⁃ jury in sepsis mice. Methods Thirty C57BL/6J mice were randomly divided into three groups：control group，LPS group，and IL-37 group，with 10 in each group. The mice in the LPS group were intraperitoneally injected with 20 mg/kg lipopolysaccharide（LPS），the mice in the IL-37 group were intraperitoneally injected with 20 mg/kg LPS and 1 mg/kg IL- 37，and the mice in the control group were intraperitoneally injected with an equal volume of sterile normal saline. The car⁃ diac function of the mice in each group was evaluated by cardiac ultrasound，and the degree of pathological damage of the myocardial tissues of the mice in each group was evaluated by HE staining. The expression levels of inflammatory factors IL-1β，IL-6 and tumor necrosis factor-α（TNF-α）in the myocardial tissues were detected by immunohistochemistry. The protein expression levels of NOD-like receptor protein 3（NLRP3）inflammasome and its related signaling pathways were an⁃ alyzed by immunohistochemistry and Western blotting. Results Compared with the control group，both left ventricle ejection fraction（LVEF）and fractional shortening（FS）in the LPS group decreased（all P<0. 01）. Compared with LPS group，both LVEF and FS in the IL-37 group increased（all P<0. 01）. Compared with the control group，the myocardial tissue structure in LPS group was disordered，with the disruptive sarcolemma，congestive and oedematous myocardial inter⁃ stitium. Compared with the LPS group，the heart function and myocardial histopathological injury were significantly im⁃ proved in the IL-37 group. The expression levels of IL-1β were 20. 12 ± 1. 89，1. 00 ± 0. 56，8. 09 ± 2. 89 in the LPS group，control group and IL-37 group，the expression levels of IL-6 were 36. 56 ± 5. 17，1. 00 ± 0. 25，9. 92 ± 1. 37，re⁃ spectively，and the expression levels of TNF-α were 20. 87 ± 6. 35，1. 00 ± 0. 44，and 6. 23 ± 1. 29，respectively. The ex⁃ pression levels of IL-1β，IL-6 and TNF-α in the myocardium in the LPS group were higher than those in the control group （all P<0. 05），and those were lower in the IL-37 group than in the LPS group（all P<0. 05）. NLRP3 protein expression levels were 6. 42 ± 0. 16 in the LPS group，1. 00 ± 0. 21 in the control group，and 5. 39 ± 0. 11 in the IL-37 group，respec⁃ tively. NLRP3 protein expression was higher in the LPS group than in the control group（P<0. 01），and NLRP3 protein ex⁃ pression was lower in the IL-37 group than in the LPS group（P<0. 05）. The relative expression levels of NLRP3 protein in the LPS group，control group and IL-37 group were 3. 77 ± 0. 22，1. 00 ± 0. 04，and 1. 97 ± 0. 13，respectively. The rela⁃ tive expression levels of Caspase-1 protein were 3. 04 ± 0. 18，1. 00 ± 0. 07，and 1. 62 ± 0. 20，respectively. The relative expression levels of ASC protein were 2. 17 ± 0. 12，1. 00 ± 0. 03 and 0. 77 ± 0. 13，respectively. The expression levels of NLRP3，Caspase-1，and ASC protein in the myocardial tissues in the LPS group were higher than those in the control group（all P<0. 05），while those were lower in the IL-37 group than in the LPS group（all P<0. 05）. Conclusions IL- 37 may ameliorate the myocardial injury in sepsis mice by inhibiting the NLRP3 inflammasome [ABSTRACT FROM AUTHOR]

Copyright of Shandong Medical Journal is the property of Shandong Medical Health Newspapers and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

To observe the expression changes of serum superoxide dismutase(SOD) and matrix metallopeptidase 9 (MMP-9) and to investigate the relationship with the degree of leukoaraiosis (LA) and the diagnosis values of serum SOD and MMP-9 forL A. Methods Seventy-eightL A patients and 30 healthy controls were selected to compare the serum SOD and MMP-9 levels between the two groups. According to Fazekas scale, LA patients were divided them into three groups: light, medium and heavy groups. The serum SOD and MMP-9 levels were compared between different groups. Pearson correlation analysis was used to analyze the correlation between serum SOD and MMP-9 levels and the degree ofL A lesions. The diagnostic efficacy of serum SOD and MMP-9 levels forL A was evaluated by receiver operating characteristic curve (ROC). Results The serum SOD level in theL A group was lower than that in the control group and the serum MMP-9 level was higher than that in the control group (both P < 0. 05). Significant difference was found in the serum SOD and MMP-9 levels among the three groups (both P <0. 05). Serum SOD level was negatively correlated with Fazekas score (r = - 0. 753, P < 0. 05). Serum MMP-9 level was positively correlated with Fazekas (r = 0. 787, P < 0. 05). The AUC of serum SOD level in diagnosis of LA was 0.8 29 and the best diagnostic cut-off value was 153. 5 U/mL While the sensitivity was 70. 5% and the specificity was 93. 3%. The AUC of serum MMP-9 level in diagnosis ofL A was 0.8 28 and the best diagnostic cut-off value was 167. 5 μg/L. While the sensitivity was 69. 2% and the specificity was 86.7 %. Conclusion The level of serum SOD decreases and MMP-9 increases inL A patients; the serum levels of SOD and MMP-9 are related to the condition ofL A and are helpful to the diagnosis ofL A . [ABSTRACT FROM AUTHOR]

Objective Using a WTX gene recombinant lentivirus interference vector to infect the mouse colorectal cancer CT26 cell line to establish a mouse colorectal cancer CT26 cell line with stable interference of WTX gene. Methods The recombinant lentiviral was transformed in mouse colorectal cancer CT26 cells, and fluorescence microscopy was used to observe the preliminary infection efficiency. The stably transfected cells were selected with (!418. The cellular expression of WTX mRNA was detected using quantitative PCR and the cellular expression of WTX protein was detected by immuno-histochemistry and Western blotting. Results After the mouse colorectal cancer CT26 cells were infected by lentiviral. the expression levels of WTX mRNA and protein were significant!) decreased in (T26 cells, indicating a mouse colorectal cancer CT26 cell line with stable interference of WTX gene was successfully established. Conclusion We successfully established a mouse colorectal cancer CT26 cell line with stable interference of WTX by lentiviral infection. [ABSTRACT FROM AUTHOR]

Objective To observe the outcome of frozen-thawed embryo transfer (FET) between the short-time and long-time culture embryos of the infertile patients whose endometria were prepared with natural or hormone replacement cycle protocol. Methods A total of 293 patients and 337 FET cycles were enrolled, and all cycles were divided into two groups depending on their means of preparing the endometria: natural cycle (NC) protocol (NC group) and hormone replacement cycle (HRT) proto-col (HRT group), each group was divided into two layers according to the culture time of thawed embryos in vitro: short-term and overnight layers. The outcomes of clinical pregnancy rate (CPR), implantation rate (IR), spontaneous abortion rate (SAR) and multiple pregnancy rate (MPR) per cycle were compared. Results In the NC and HRT groups, the IR was respectively 32.69%and 21.83%, the CPR was 59.15%and 41.73%, the SAR was 7.14%and 16.22%, the MPR was 21.43%and 13.51%, respectively, and the IR and CPR were all higher in the NC group than those of the HRT group (all P<0.05).In the short-term and overnight layers of the NC group, the IR was 28.13%and 35.87%, the CRP was 53.57%and 62.79%, the SAR was 6.67%and 7.41%, and the MPR was 20.00%and 22.22%, respectively; no significant differences were found between the two groups. Similarly, in the HRT group's different culture time layers, the IR was 21.92%and 21.74%, the CRP was 44.19%and 39.42%, the SAR was 21.05%and 11.11%, and the MPR was 12.28%and 14.81%, respectively; no statistically significant difference was found. In short-term layer, the outcomes of FET were not statistically different between the NC group and HRT group. Compared with the overnight layer of HRT group, IR and CPR were all higher than in the overnight layer of NC group (all P<0.05).Conclusion Endometrium prepared with NC protocol can obtain a better FET outcome than that of the HRT protocol, which mainly embodied in the selection of the thawed embryos' culture time in vitro before transplant, and culture overnight is appropriate. [ABSTRACT FROM AUTHOR]

目的观察原发免疫性血小板减少症（ITP)小鼠外周血CD4+ T细胞程序性死亡分子1 (PD-1)表达及血 清IFN-γ, IL-10水平情况。方法 20只Balb/c小鼠随机分为正常组、模型组各10只。正常组不做特殊处理，模型 组隔日给予腹腔注射豚鼠抗小鼠血小板抗血清（GP-APS) 100 μL/20g，建立ITP模型，造模成功后继续隔日注射 GP-APS,连续7 d。第8天取两组小鼠外周血，流式细胞术检测CD4+ T细胞表面PD-1的表达，同时分离血清，用 CBA法检测IFN-y、L-10水平。结果模型组、正常组小鼠外周血CD4+ T细胞PD-1表达率分别为8. 16% ± 1.03%82% ±0. 38% ,两组相比P <0. 05。模型组、正常组小鼠血清IFN-y水平分别为（3. 58 ± 0. 68)、（1.42 ± 0.61) pg/mL, IL-10 水平分别为（18. 25 ± 1.94)、（27.66 ±4 98) pg/mL,两组相比 P 均 < 0.05。结论 ITP 小鼠外周 血CD4+ T细胞表面PD-1表达增高，血清IFN-y水平升高，血清IL-10水平降低，ITP疾病中存在Th1/Th2失衡。 [ABSTRACT FROM AUTHOR]

Copyright of Shandong Medical Journal is the property of Shandong Medical Health Newspapers and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

目的 观察替格瑞洛口服对合并糖尿病的不稳定型心绞痛经皮冠状动脉介入术（ＰＣＩ）治疗患者血清炎症因子水平的影响。方法 １２４例合并糖尿病的不稳定型心绞痛患者随机分为观察组和对照组各６２例，两组均 行ＰＣＩ。观察组术前３ｄ口服负荷剂量（１８０ｍｇ）替格瑞洛，随后给予维持剂量（９０ｍｇ），２次／ｄ，维持６个月；对照组术前３ｄ口服负荷剂量（３００ｍｇ）氯吡格雷，随后给予维持剂量（７５ｍｇ），１次／ｄ，维持６个月；其余治疗措施相同。 比较两组手术前后外周血循环内皮细胞（ＣＥＣ）及血清ＣＤ４０配体（ＣＤ４０Ｌ）、ＩＬ-６、高敏Ｃ反应蛋白（ｈｓ-ＣＲＰ）。结果 与同组术前比较，两组术后１２ｈ的ＣＥＣ增加，ＣＤ４０Ｌ、ＩＬ-６、ｈｓ-ＣＲＰ水平升高；与同组术后１２ｈ比较，两组术后１ 个月ＣＥＣ减少，ＣＤ４０Ｌ、ＩＬ-６、ｈｓ-ＣＲＰ水平降低；与对照组比较，观察组术后１、６个月ＣＥＣ减少，ＣＤ４０Ｌ、ＩＬ-６、ｈｓ-ＣＲＰ 水平降低；Ｐ均＜０．０５。结论 合并糖尿病的不稳定型心绞痛ＰＣＩ治疗患者替格瑞洛应用后血清炎症因子水平下降，其对血管起到保护作用。 [ABSTRACT FROM AUTHOR]

自噬作为一种细胞应对代谢应激的代偿机制，能使肿瘤细胞适应代谢应激，阻止细胞凋亡、促其存活，表皮生长因子受体（ EGFR）通路在自噬功能调节中发挥一定作用。自噬与表达 EGFR 肿瘤的发生发展有关，早期可抑制细胞癌变，在成熟的肿瘤细胞中则可维持其存活、抑制凋亡。自噬还参与了 EGFR 靶向药物耐药性形成，抑制自噬可显著增加 EGFR 靶向治疗的敏感性。 [ABSTRACT FROM AUTHOR]

目的　根据老年高血压患者的个体情况采取针对性的联合降压药物及生物反馈治疗，观察其疗效。方法　将５０３例老年高血压患者随机分成对照组和观察组，对照组给予常规降压治疗，观察组在此基础上根据血清同型半胱氨酸（Ｈｃｙ）、微量蛋白尿水平和焦虑抑郁评分结果给予叶酸、阿托伐他汀和（或）生物反馈治疗。比较两组血压达标情况、Ｈｃｙ及尿微量白蛋白水平、焦虑抑郁评分，观察１年后主要不良事件（ＭＡＣＥ）发生率。结果　治疗１年后，观察组坐位血压、血清Ｈｃｙ、尿微量白蛋白及焦虑抑郁评分均低于对照组，Ｐ均＜０．０１。观察组血压达标２３９例（９５．６％）、达标时间（６．２±２．４）个月，对照组分别为２１２例（８５．５％）、（８．３±２．１）个月，Ｐ均＜０．０１。观察组ＭＡＣＥ发生率（８．４％）明显低于对照组（１６．９％），Ｐ＜０．０１。结论　根据老年高血压患者的Ｈｃｙ水平、微量白蛋白尿水平、焦虑抑郁评分给予个体化治疗及生物反馈治疗，可有效降低血压，保护靶器官。 [ABSTRACT FROM AUTHOR]