1. Capillary zone electrophoresis method for determination of bitter (α- and β-) acids in hop (Humulus lupulus L.) cone extracts.
- Author
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Kornyšova, O., Stanius, Ž., Obelevičius, K., Ragažinskienė, O., Skrzydlewska, O., and Maruška, A.
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CAPILLARY electrophoresis , *ELECTROPHORESIS , *ORGANIC acids , *PLANT extracts , *HOPS , *BITTERNESS (Taste) , *MEDICINAL plants - Abstract
Purpose: Humulus lupulus (H. lupulus), more commonly known as hop, is a member of the Cannabaceae family with male and female flowers on separate plants. It is native in Europe including Lithuania, Asia and North America. Hop has been recognized as a medicinal plant for centuries, nevertheless different medicinal activities Of hop are currently investigated and discovered. An important class of hop compounds is the hop acids, which are classified as α-acids and β-acids. Different varieties of hops vary in amount and composition of hop acids. Methods: Simple capillary zone electrophoresis method has been optimized and applied for the analysis of hop acids in hop cone extracts. Results: With this method the analysis takes ca. 10 min. Repeatability for migration times and peak areas expressed as relative standard deviation were up to 0.21% and 5.96%, respectively. Conclusions: Comparative results of capillary zone electrophoretic analysis of extracts of different hop varieties and conductometric titration, as a standard method for determination of α-acids, are presented. Both methods provide consistent results, however capillary zone electrophoresis is capable of separating co, form of humulones from other forms. [ABSTRACT FROM AUTHOR]
- Published
- 2009
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