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Made available in DSpace on 2022-04-28T20:51:54Z (GMT). No. of bitstreams: 0 Previous issue date: 2009-05-29 Hepatitis C is transmitted primarily parenterally by contaminated blood and is often associated with: intravenous drug abuse, invasive procedures, blood transfusions, acupuncture, tattooing, and alcohol and tobacco use. This study aimed to quantify and evaluate the risk factors among blood donors, volunteer blood donors and replacement individuals, infected or not by the C virus. The main transmission routes of C virus were identified in 55 men and 25 women (GI) monitored by the Ambulatory Unit of the Department of Tropical Diseases, Botucatu Medical School, and in 24 men and 26 women (GII), all active blood donors at the Bauru State Hospital Transfusional Agency. Both groups were similar in: tobacco and alcohol consumption, sexual behavior, tattooing and illicit drug use. The duration of alcohol and tobacco consumption and blood transfusions in GI were longer, whereas the option for steady partners, condom use, disposable materials and piercings were predominant in GII. In conclusion, the risk factors for hepatitis C demonstrate the necessity of health policies that act on the primary and secondary prevention levels (respectively, reduction of infection incidence and hepatopathy risk). Bauru State Hospital Botucatu Medical School São Paulo State University, UNESP, Bauru, São Paulo State Department of Tropical Diseases Botucatu Medical School São Paulo State University, UNESP, Botucatu, São Paulo State Assessoria de Planejamento do Hospital Estadual Bauru Universidade Estadual Paulista, UNESP, Av. Eng. Luis Edmundo Carrijo Coube, 1-100, Bauru, SP, 17.033-360 Bauru State Hospital Botucatu Medical School São Paulo State University, UNESP, Bauru, São Paulo State Department of Tropical Diseases Botucatu Medical School São Paulo State University, UNESP, Botucatu, São Paulo State Assessoria de Planejamento do Hospital Estadual Bauru Universidade Estadual Paulista, UNESP, Av. Eng. Luis Edmundo Carrijo Coube, 1-100, Bauru, SP, 17.033-360

Made available in DSpace on 2013-08-12T18:00:28Z (GMT). No. of bitstreams: 0 Previous issue date: 2008-01-01 Made available in DSpace on 2013-09-30T18:38:06Z (GMT). No. of bitstreams: 0 Previous issue date: 2008-01-01 Submitted by Vitor Silverio Rodrigues (vitorsrodrigues@reitoria.unesp.br) on 2014-05-20T13:34:27Z No. of bitstreams: 0 Made available in DSpace on 2014-05-20T13:34:27Z (GMT). No. of bitstreams: 0 Previous issue date: 2008-01-01 Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) A cross-sectional study was performed on HIV-1 infected individuals with or without antiretroviral treatment (ARV) in the AIDS Day Hospital, Botucatu Medical School, UNESP. Between August 2004 and October 2005, 73 HIV-1 infected individuals were divided into three groups: infected individuals with or without AIDS who had never received ARV (G(1) = 15); patients on HAART that had had plasma HIV-1 RNA viral load (VL) equal to or greater than 50 copies/mL (G(2) = 27); and patients on HAART with undetectable VL for at least the past six months (G(3) = 31). There was also an additional group that comprised blood donors without any sign of the disease and with negative HIV serum tests (G(4) = 20), which was the control group. Serum cytokine levels (values in pg/mL) were measured by enzyme-linked immunosorbent assay (ELISA) and specific mRNA expression by reverse transcription polymerase chain reaction (RT-PCR). Both techniques were performed on the four groups for TNF-alpha, IL-2, INF-gamma, IL-4 and IL-10. All patients were submitted to VL determination and CD4(+) and CD8(+) T lymphocyte counts. The analysis of the results revealed a significant comparison among groups for both methods and an association between the latter (>80% - r(2) > 0.80). There was only one exception, in control individuals for IL-2 by ELISA. The cytokine profiles, in both methods, for the three patient groups, were mature Th-0. The behaviors of IL-2 and INF-gamma required emphasis due to consequent expression of dominant Th profile. Both methods showed low IL-2 and high mean values of INF-gamma in the three groups. Several authors have recently drawn attention to the substantial apoptosis of infected and non-infected CD4(+) T cells, mainly during primary infection, persisting only in those with INF-gamma phenotype producer and not IL-2. HIV infected individuals submitted to HAART are expected to produce IL-2 in an attempt to present Th-1 profile, but in most cases this did not occur. São Paulo State Univ, UNESP, Botucatu Med Sch, Dept Trop Dis, Botucatu, SP, Brazil São Paulo State Univ, UNESP, Botucatu Biosci Inst, Dept Microbiol & Immunol, Botucatu, SP, Brazil São Paulo State Univ, UNESP, Botucatu Med Sch, Dept Trop Dis, Botucatu, SP, Brazil São Paulo State Univ, UNESP, Botucatu Biosci Inst, Dept Microbiol & Immunol, Botucatu, SP, Brazil

Submitted by Vitor Silverio Rodrigues (vitorsrodrigues@reitoria.unesp.br) on 2014-05-27T11:22:02Z No. of bitstreams: 0Bitstream added on 2014-05-27T14:40:13Z : No. of bitstreams: 1 2-s2.0-33846294386.pdf: 286370 bytes, checksum: 7fdca13ec6a50f7e9f657a080ff0e3d0 (MD5) Made available in DSpace on 2014-05-27T11:22:02Z (GMT). No. of bitstreams: 0 Previous issue date: 2006-11-01 Laboratory profile of young ovines was studied in order to evaluate and compare their antiserum production from natural and Cobalt-60 irradiated Crotalus durissus terrificus (C.d.t.) venoms. The parameters analyzed included complete blood count, and urea, creatinine, aspartate aminotransferase, total proteins, albumin and globulin serum measurements. Three groups of six animals each were used. Group 1 (G1) received natural C.d.t. venom; Group 2 (G2) received irradiated C.d.t. venom; and Group 3 (G3) was used as control and did not receive venom, only adjuvants, using seven venom inoculations. During the experimental period, animals were fortnightly weighed. According to clinical and weight evaluation, sheep in post-weaning phase showed no changes in their physiological profiles but had excellent weight gain. The parameters analyzed were not statistically different (p