1. Adult exposure to sasanguasaponin induces spermatogenic cell apoptosis in vivo through increased oxidative stress in male mice.
- Author
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Jian Li, Zixu Wang, Dazhao Shi, and Yaoxing Chen
- Subjects
APOPTOSIS ,OXIDATIVE stress ,LABORATORY mice ,CAMELLIA oleifera ,PLANT extracts ,BODY weight ,MALONDIALDEHYDE ,SEMEN ,DRUG dosage - Abstract
Effects of sasanguasaponin (SGS), a compound from defatted seeds of Camellia oleifera, on abnormal spermatogenesis were studied in adult male mice using daily intragastric administration of 100, 200 and 400 mg/kg body weight (BW) for 6 weeks. The sperm counts decreased and the number of abnormal spermatozoa increased in mice treated with SGS at 400 mg/kg dose level. Testicular weight and seminiferous tubular area gradually decreased as dosage increased to 400 mg/kg SGS. Germ cells were rarefied and showed irregular distribution in seminiferous tubules (STs). Apoptosis was pronounced among spermatocytes and spermatids at the 400 mg/kg dose level. Antioxidant enzymes including superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) and total antioxide capacity (T-AOC) markedly decreased and malondialdehyde (MDA) concentration increased in the testes. These results suggest that adult exposure to SGS (400 mg/kg) induces spermatogenic apoptosis through increased oxidative stress in male mice. [ABSTRACT FROM AUTHOR]
- Published
- 2010
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