1. ELISA-like Analysis of Cisplatinated DNA Using Magnetic Separation.
- Author
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Smerkova K, Vlcnovska M, Dostalova S, Milosavljevic V, Kopel P, Vaculovic T, Krizkova S, Vaculovicova M, Adam V, and Kizek R
- Abstract
Cisplatin belongs to the most widely used cytostatic drugs. The determination of the presence of the DNA-cisplatin adducts may not only signal the guanine-rich regions but also monitor the interaction reaction between DNA and the drug in terms of speed of interaction. In this work, the combined advantages of magnetic particles-based isolation/purification with fluorescent properties of quantum dots (QDs) and antibodies targeted on specific recognition of DNA-cisplatin adducts are demonstrated. The formation of a complex between magnetic particles with surface modified by anti-dsDNA antibody, cisplatin-modified DNA and QDs labelled anti-cisplatin-modified DNA antibody was suggested and optimized., Competing Interests: The authors declare no conflicts of interest.
- Published
- 2015
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