1. Experimental Infection of Subpopulations of Human Peripheral Blood Leukocytes by Herpes Simplex Virus
- Author
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Susan Plaeger-Marshall and Jerry W. Smith
- Subjects
T-Lymphocytes ,HSL and HSV ,In Vitro Techniques ,Lymphocyte Activation ,Virus Replication ,medicine.disease_cause ,Monocytes ,General Biochemistry, Genetics and Molecular Biology ,Virus ,Cell Line ,Leukocytes ,medicine ,Humans ,Simplexvirus ,Polymorphonuclear leukocyte ,B-Lymphocytes ,biology ,Inoculation ,Cell Transformation, Viral ,Virology ,Peripheral blood ,Herpes simplex virus ,Lymphoblastoid cell ,Immunology ,biology.protein ,Mitogens ,Antibody - Abstract
SummaryResults from examination of peripheral blood leukocytes from five subjects, three with antibody to HSV and two without, showed that HSV type 1 may replicate in mitogen-stimulated T and B lymphocytes and in continuous lymphoblastoid cell lines derived from either B or T cells. Significant replication was also noted in monocytes from each subject but not in polymorphonuclear leukocyte subpopulations. Replication in monocytes was found to be dependent on certain conditions of culture and viral inoculation. Only adherent cells cultured for 72-hr prior to infection produced significant yields of virus.
- Published
- 1978
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