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Your search keyword '"Romano, Marta"' showing total 14 results
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14 results on '"Romano, Marta"'

1. The Influence of Holder Pasteurization on the Diversity of the Human Milk Bacterial Microbiota Using High-Throughput DNA Sequencing

Author

García-González, Igrid, primary, Corona-Cervantes, Karina, additional, Hernández-Quiroz, Fernando, additional, Villalobos-Flores, Loan Edel, additional, Galván-Rodríguez, Flor, additional, Romano, Marta Catalina, additional, Miranda-Brito, Carolina, additional, Piña-Escobedo, Alberto, additional, Borquez-Arreortúa, Francisco Guillermo, additional, Rangel-Calvillo, Martín Noé, additional, and García-Mena, Jaime, additional

Published
2021
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14. The Influence of Holder Pasteurization on the Diversity of the Human Milk Bacterial Microbiota Using High-Throughput DNA Sequencing.

Author

García-González I, Corona-Cervantes K, Hernández-Quiroz F, Villalobos-Flores LE, Galván-Rodríguez F, Romano MC, Miranda-Brito C, Piña-Escobedo A, Borquez-Arreortúa FG, Rangel-Calvillo MN, and García-Mena J

Subjects
Bacteria genetics, Breast Feeding, Cross-Sectional Studies, Female, High-Throughput Nucleotide Sequencing, Humans, Infant, Milk, Human microbiology, Pasteurization, RNA, Ribosomal, 16S genetics, Microbiota genetics, Milk Banks
Abstract

Background: Human milk is the best food for infants; however, when breastfeeding is not possible, pasteurized milk from human milk banks is the best alternative. Little has been reported about variations in the bacterial microbiota composition of human milk after pasteurization., Research Aim: To characterize and compare the bacterial microbiota composition and diversity within human milk among Mexican mothers before and after the Holder pasteurization process., Methods: A cross-sectional, observational, and comparative design was used. The effect of the pasteurization process on the bacterial composition and diversity of human milk samples of donors ( N = 42) from a public milk bank was assessed before and after pasteurization by high throughput deoxyribonucleic acid sequencing of V3-16S rRNA gene libraries. Sequencing data were examined using the Quantitative Insights into Microbial Ecology software and Phyloseq in R environment., Results: A varied community of bacteria was found in both raw and pasteurized human milk. The bacterial diversity of the milk samples was increased by the pasteurization, where some thermoduric bacteria of the phyla Proteobacteria, Firmicutes , and Actinobacteria were more abundant. The source tracker analysis indicated that at most 1.0% of bacteria may have come from another source, showing the safety of the process used to treat milk samples., Conclusion: The pasteurization process increased the bacterial diversity. We selected taxa capable of surviving the process, which could proliferate after the treatment without being a risk for infants.

Published
2022
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