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SummaryThe 3 species of Trichomonas do not show any significant difference in survival time in the prostate gland if they have had previous animal passage. It is interesting to note that without previous passage T. buccalis and T. vaginalis closely resemble each other in their survival time. This is in accordance with the opinions of several workers who have pointed out the morphological similarities of these two species.Although it obviously cannot be assumed that conditions in the prostate gland of the rat and of man are identical, these experiments do indicate that trichomonads can survive for some time in this type of tissue. Furthermore, by making cultures of the expressed prostatic fluid the incidence of T. vaginalis may prove to be greater than present statistics indicate.