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Your search keyword '"C-C motif chemokine ligand 5"' showing total 2 results
Start Over Descriptor "C-C motif chemokine ligand 5" Publisher sage publications
2 results on '"C-C motif chemokine ligand 5"'

1. miR-147a suppresses the metastasis of non-small-cell lung cancer by targeting CCL5

Author

Xiao Rong Luan and Yan Lu

Subjects
0301 basic medicine, Chemokine, Lung Neoplasms, NSCLC, migration, Biochemistry, CCL5, Metastasis, Pre-Clinical Research Report, 03 medical and health sciences, Mice, 0302 clinical medicine, Downregulation and upregulation, miR-147a, In vivo, Cell Movement, Carcinoma, Non-Small-Cell Lung, Cell Line, Tumor, microRNA, medicine, metastasis, Animals, Humans, Lung cancer, Chemokine CCL5, Cell Proliferation, C-C motif chemokine ligand 5, biology, Cell growth, business.industry, Biochemistry (medical), Cell Biology, General Medicine, medicine.disease, invasion, respiratory tract diseases, MicroRNAs, 030104 developmental biology, non-small-cell lung cancer, 030220 oncology & carcinogenesis, biology.protein, Cancer research, business, Neoplasm Transplantation
Abstract

Objective MicroRNA (miR)-147a acts as an inhibitory miRNA in many cancers. However, its potential roles in non-small-cell lung cancer (NSCLC) remain unclear. Methods Levels of miR-147a and C-C motif chemokine ligand 5 (CCL5) were measured using a quantitative real-time PCR assay. Cell growth, migration, and invasion of NSCLC cells were assessed by colony formation, wound healing, and Transwell invasion assays, respectively. The role of miR-147a in the growth and metastatic ability of NSCLC in vivo was detected using a xenograft model and experimental lung metastasis model. Results miR-147a was downregulated in NSCLC cell lines as well as in tissues. Gain-of-function and loss-of-function analyses demonstrated that upregulation of miR-147a decreased the aggressiveness of NSCLC cells in vitro. In addition, CCL5 was identified as a target of miR-147a. We also demonstrated the effect of miR-147a in the progression of NSCLC cells via targeting CCL5. Finally, the in vivo mouse xenograft model showed that miR-147a inhibited progression of NSCLC cells. Conclusions Overall, expression of miR-147a was downregulated in NSCLC. Importantly, upregulation of miR-147a suppressed the growth and metastasis of NSCLC cells in vivo by targeting CCL5.

Published
2019

2. miR-147a suppresses the metastasis of non-small-cell lung cancer by targeting CCL5 .

Author

Lu Y and Luan XR

Subjects
Animals, Cell Line, Tumor, Cell Movement, Cell Proliferation, Humans, Mice, Neoplasm Transplantation, Carcinoma, Non-Small-Cell Lung genetics, Chemokine CCL5, Lung Neoplasms genetics, MicroRNAs genetics
Abstract

Objective: MicroRNA (miR)-147a acts as an inhibitory miRNA in many cancers. However, its potential roles in non-small-cell lung cancer (NSCLC) remain unclear., Methods: Levels of miR-147a and C-C motif chemokine ligand 5 (CCL5) were measured using a quantitative real-time PCR assay. Cell growth, migration, and invasion of NSCLC cells were assessed by colony formation, wound healing, and Transwell invasion assays, respectively. The role of miR-147a in the growth and metastatic ability of NSCLC in vivo was detected using a xenograft model and experimental lung metastasis model., Results: miR-147a was downregulated in NSCLC cell lines as well as in tissues. Gain-of-function and loss-of-function analyses demonstrated that upregulation of miR-147a decreased the aggressiveness of NSCLC cells in vitro . In addition, CCL5 was identified as a target of miR-147a. We also demonstrated the effect of miR-147a in the progression of NSCLC cells via targeting CCL5. Finally, the in vivo mouse xenograft model showed that miR-147a inhibited progression of NSCLC cells., Conclusions: Overall, expression of miR-147a was downregulated in NSCLC. Importantly, upregulation of miR-147a suppressed the growth and metastasis of NSCLC cells in vivo by targeting CCL5.

Published
2020
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