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1. Radioimmunoassay for pancreatic glucagon based on specific derivatisation of the hormone.

Author

Nooijen WJ and Koppert PW

Subjects
Adult, Animals, Aprotinin, Arginine, Drug Stability, Fasting, Glucagon analogs & derivatives, Glucagon immunology, Glucose Tolerance Test, Humans, Middle Aged, Rabbits immunology, Radioimmunoassay methods, Glucagon blood, Pancreas metabolism
Abstract

A specific glucagon antiserum was generated in rabbits using a derivatisation of the methionine residue in glucagon. A sensitive and specific assay for glucagon has been developed. Interference with the assay system by plasma factors was abolished by both extraction of plasma samples and the production of glucagon-free plasma for each individual.

Published
1981
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