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Kitoza is a traditional salted and dried and/or smoked meat product of Madagascar. lt is made from beef or pork strips and is produced at artisanal and familial levels. In a previous study, the analyses of 60 end-products showed that smoked Kitoza contained approximately SO g/100g of water, 3g/100g of salt and showed a water activity of 0.93 on average. They are thus classified mainly in food with high moisture content while the most dried products are in the zone of intermediate humidity food (Leistner and Rödel, 1976). Smoking the product however led to Benzo (a) Pyrene (B(a)P) content {indicator of carcinogenic compounds contamination of cooked and Smoked meat products) above the norm of 5 ppb in 10 samples. Moreover, if the final pH values (of the order of 5.8) indicated that Kitoza is not a fermented food, 11 samples had some D-lactic acid content as described in sausage, a well-known fermented product. This study describes the traditional process for making smoked Kitoza. This process has not been the focus of any other scientific study to date.It has been characterized in terms of mass transfers during salting and smoking (salt gain, water loss) and evolution of biochemical (water, salt, Aw, pH, titrable acidity, D andLl-lactic acid , phenol and B(a)P contents) and microbiological (lactic acid bacteria and total flora) characteristics during the process. Measurements were performed on the raw material, the product after salting, and the product after smoking. This kinetic study allows defining better the unit operations involved in the process and their impact on product quality.

Kitoza is a traditional product from Madagascar manufactured either with strips of pork or beef meat. lt is an artisanal product manufactured in rural and urban regions. The first step of the process is salting with coarse salt mixed with spices and then either a drying or smoking step is carried out. Samples from pork and beef and both processes have been analysed. The microbiological analyses revealed the process allowed the selection of microorganisms with potential technological interest. Thus a high level of coagulase negative staphylococci (CNS) was noticed: between 5 to7 log CFU/g. These technological bacteria seemed well adapted to the two processes drying or smoking. 811 isolates of presumed CNS from Manitol Salt agar have been identified. Two approaches have been applied: a PCR multiplex as developed by Corbière Morot-Bizot et al. (2004. J. Appl. Microbial. 97, 1087-1094) or a staph array developed by Giammarinaro et al. (2005. J. Clin. Microbiol., 3673-3680) allowing the identification of 36 CNS species. A total of 9 species of CNS were identified in the Kitoza with 7 species for the beef and 8 for the pork meats. Staphylococcus saprophyticus was the dominant species in all the products and the major one in dried pork and beef smoked or dried. While in smoked pork, in addition of S. saprophyticus (50%), S. xy/osus (13%), S. equorum (15%), S. succinus (13%) and S. epidermidis (9%) were identified. This study highlighted that the process: salting and drying or smoking allowed the selection of coagulase negative staphylococci. These CNS are well described in the literature as contributing to the quality of meat products, with some species such as S. xylosus already used as starter cultures for the manufacture of meat products. This work was funded by EU, 7th Framework Programme, AFTER project (grant agreement 245025).

Kitoza is a traditional product from Madagascar manufactured either with strips of pork or beef meat. lt is an artisanal product manufactured in rural and urban regions. The first step of the process is salting with coarse salt mixed with spices and then either a drying or smoking step is carried out. Samples from pork and beef and both processes have been analysed. The microbiological analyses revealed the process allowed the selection of microorganisms with potential technological interest. Thus a high level of coagulase negative staphylococci (CNS) was noticed: between 5 to7 log CFU/g. These technological bacteria seemed well adapted to the two processes drying or smoking. 811 isolates of presumed CNS from Manitol Salt agar have been identified. Two approaches have been applied: a PCR multiplex as developed by Corbière Morot-Bizot et al. (2004. J. Appl. Microbial. 97, 1087-1094) or a staph array developed by Giammarinaro et al. (2005. J. Clin. Microbiol., 3673-3680) allowing the identification of 36 CNS species. A total of 9 species of CNS were identified in the Kitoza with 7 species for the beef and 8 for the pork meats. Staphylococcus saprophyticus was the dominant species in all the products and the major one in dried pork and beef smoked or dried. While in smoked pork, in addition of S. saprophyticus (50%), S. xy/osus (13%), S. equorum (15%), S. succinus (13%) and S. epidermidis (9%) were identified. This study highlighted that the process: salting and drying or smoking allowed the selection of coagulase negative staphylococci. These CNS are well described in the literature as contributing to the quality of meat products, with some species such as S. xylosus already used as starter cultures for the manufacture of meat products. This work was funded by EU, 7th Framework Programme, AFTER project (grant agreement 245025).

Kitoza is a traditional salted and dried and/or smoked meat product of Madagascar. lt is made from beef or pork strips and is produced at artisanal and familial levels. In a previous study, the analyses of 60 end-products showed that smoked Kitoza contained approximately SO g/100g of water, 3g/100g of salt and showed a water activity of 0.93 on average. They are thus classified mainly in food with high moisture content while the most dried products are in the zone of intermediate humidity food (Leistner and Rödel, 1976). Smoking the product however led to Benzo (a) Pyrene (B(a)P) content {indicator of carcinogenic compounds contamination of cooked and Smoked meat products) above the norm of 5 ppb in 10 samples. Moreover, if the final pH values (of the order of 5.8) indicated that Kitoza is not a fermented food, 11 samples had some D-lactic acid content as described in sausage, a well-known fermented product. This study describes the traditional process for making smoked Kitoza. This process has not been the focus of any other scientific study to date.It has been characterized in terms of mass transfers during salting and smoking (salt gain, water loss) and evolution of biochemical (water, salt, Aw, pH, titrable acidity, D andLl-lactic acid , phenol and B(a)P contents) and microbiological (lactic acid bacteria and total flora) characteristics during the process. Measurements were performed on the raw material, the product after salting, and the product after smoking. This kinetic study allows defining better the unit operations involved in the process and their impact on product quality.