4 results on '"Maria Rosa Ciardi"'
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2. Tubular Epithelium Culture from Nephronophthisis-Affected Kidneys: A New Approach to Molecular Disorders of Tubular Cells
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Rosanna Gusmano, Adalberto Sessa, Giovanni Candiano, Roberta Bertelli, Gian Marco Ghiggeri, Laura Torri Tarelli, Maria Rosa Ciardi, Mietta Meroni, and Fabrizio Ginevri
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Cell Extracts ,medicine.medical_specialty ,Biopsy ,Cytokeratin ,Tissue culture ,Laminin ,Internal medicine ,medicine ,Humans ,Electrophoresis, Gel, Two-Dimensional ,Child ,Cells, Cultured ,Kidney ,biology ,medicine.diagnostic_test ,Molecular biology ,In vitro ,Microscopy, Electron ,Kidney Tubules ,medicine.anatomical_structure ,Endocrinology ,Nephrology ,biology.protein ,Collagenase ,Nephritis, Interstitial ,Alkaline phosphatase ,Peptides ,medicine.drug - Abstract
Abnormalities of tubular membrane structure and composition have been proposed as the primary defect in nephronophthisis (NEF). In order to characterize the protein composition of tubular cells in NEF, in vitro methods were developed to culture and propagate tubular cells obtained from biopsy fragments. Accordingly, microdissected cortical slices (1 x 3 mm) were first digested with collagenase and DNAse and then grown in RPMI medium supplemented with 10% NU serum and conditioned serum deriving from 3T3 cultures. At confluence, cultured cells from NEF showed characteristics which were typical of normal tubules, i.e. presence of cytokeratin and positivity for succinic dehydrogenase and alkaline phosphatase stainings, and presented no morphological alterations compared to cultured cells from normal tubular epithelium. Moreover, no difference was observed for fibronectin, collagen IV and laminin stains. Analysis by two-dimensional electrophoresis of cellular extracts revealed several changes in protein composition of NEF, the main one being the decrease in NEF cells of a polypeptide with a molecular weight of 120 kD and a pI of 4.8; this polypeptide was a constant finding in normal kidneys. These observations demonstrated that human tubular epithelial cells can be successfully cultured from very small biopsy fragments, which represents a new approach to the study of molecular disorders involving tubular cells in inherited disease. Cultured cells from NEF maintain the same morphological, immunological and cytochemical characteristics as normal tubular cells, but present a few alterations in polypeptide composition which may have pathogenetic relevance. A more careful analysis of these alterations is needed to define the molecular disorder(s) involving the tubule in NEF.
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- 1990
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3. Contents, Vol. 10, 1990
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Shaul G. Massry, Gilbert Deray, Fumiaki Marumo, Gerald A. Young, Barbara F. Prowant, Michèle Dubois, Joel D. Kopple, Richard Bourbouze, F. Martinez, Roberta Bertelli, Corrine Algrim, Esteban Poch, Ronaldo R. Bergamo, Alejandro Darnell, Rosanna Gusmano, Carlos Quereda, J. Grellet, B. Baumelou, Maria Rosa Ciardi, Bertr Baumelou, Christy A. Price, Adalberto Sessa, Esa Soppi, C. Jacobs, Janet Dichiro, Larry A. Slomowitz, Fabrizio Ginevri, Jukka Mustonen, Joaquín Ortuño, Francis Hon-wai Wong, Mary Grosvenor, Albert Torras, Miguel González-Clemente, Mariana Linker-Israeli, Amedeo F. De Vecchi, Alek M. Brownjohn, Laura Torri Tarelli, Edward F. Vonesh, Claude Jacobs, Gary M. Rabetoy, Zbigniew Gaciong, Masayoshi Shichiri, Kiyohide Fushimi, Maite Rivera, Patricia Ho, Patrick Lau, John W.A. Findlay, Luis Revert, Mietta Meroni, Olavi Hällström, Amos Pasternack, J.-P. Rey, Jeffrey M. Sailstad, Jadwiga M. Alexiewicz, K. K. Pun, Hélène Beaufils, Marian Klinger, G. Deray, Thomas O. Pitts, Koichi Matsumoto, José, Alain Baumelou, Gian Marco Ghiggeri, Vinay Sakhuja, H. Boulechfar, Allen R. Nissenson, Kirpal S. Chugh, A. Botey, Claudia Castelnovo, Ana Gonzalo, Giovanni Candiano, Karl D. Nolph, and M.F. Bellin
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Gerontology ,Nephrology ,business.industry ,Library science ,Medicine ,business - Published
- 1990
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4. Subject Index, Vol. 10, 1990
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Gilbert Deray, J. Grellet, Michèle Dubois, Barbara F. Prowant, Hélène Beaufils, J.-P. Rey, Karl D. Nolph, Jadwiga M. Alexiewicz, Masayoshi Shichiri, Patricia Ho, Mietta Meroni, M.F. Bellin, Claude Jacobs, Kiyohide Fushimi, Alain Baumelou, Amos Pasternack, Ronaldo R. Bergamo, Mariana Linker-Israeli, Laura Torri Tarelli, Rosanna Gusmano, Edward F. Vonesh, O. Hällström, Gary M. Rabetoy, Esteban Poch, Gian Marco Ghiggeri, Luis Revert, Jeffrey M. Sailstad, Fabrizio Ginevri, Carlos Quereda, K. K. Pun, Maria Rosa Ciardi, Mary Grosvenor, Bertr Baumelou, Alek M. Brownjohn, Christy A. Price, E. Soppi, Thomas O. Pitts, Francis Hon-wai Wong, José, Richard Bourbouze, Miguel González-Clemente, Patrick Lau, Alejandro Darnell, John W.A. Findlay, Kirpal S. Chugh, Jukka Mustonen, Shaul G. Massry, H. Boulechfar, Marian Klinger, Allen R. Nissenson, Claudia Castelnovo, Fumiaki Marumo, Gerald A. Young, Albert Botey, Ana Gonzalo, Vinay Sakhuja, Giovanni Candiano, G. Deray, Joel D. Kopple, F. Martinez, Maite Rivera, Koichi Matsumoto, Corrine Algrim, Roberta Bertelli, Zbigniew Gaciong, B. Baumelou, Adalberto Sessa, Albert Torras, C. Jacobs, Janet Dichiro, Larry A. Slomowitz, Joaquín Ortuño, and Amedeo F. De Vecchi
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Gerontology ,Index (economics) ,Nephrology ,business.industry ,Medicine ,Subject (documents) ,business - Published
- 1990
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