1. Mobilization of Bone Marrow Cells by CSF3 Protects Mice from Bleomycin-Induced Lung Injury
- Author
-
Rongxuan Zhang, Yuan Zhang, Xueyuan Chen, Han-shui Jiang, Hui Zheng, Lu Zhang, Fen Zhang, Jian-xin Chu, Xue-jin Chen, and Huiping Li
- Subjects
Pulmonary and Respiratory Medicine ,Pathology ,medicine.medical_specialty ,Pulmonary Fibrosis ,Transgene ,Green Fluorescent Proteins ,Bone Marrow Cells ,Mice, Transgenic ,Lung injury ,Mesenchymal Stem Cell Transplantation ,Bleomycin ,Gene Expression Regulation, Enzymologic ,Flow cytometry ,Mice ,chemistry.chemical_compound ,Granulocyte Colony-Stimulating Factor ,medicine ,Animals ,Regulation of gene expression ,Antibiotics, Antineoplastic ,Mobilization ,medicine.diagnostic_test ,business.industry ,Bone Marrow Stem Cell ,Flow Cytometry ,Matrix Metalloproteinases ,Recombinant Proteins ,Mice, Inbred C57BL ,medicine.anatomical_structure ,chemistry ,Female ,Bone marrow ,business - Abstract
Background: Bone marrow-derived cells may play a role in tissue injury and repair. Growth factors facilitate the mobilization of bone marrow-derived cells to the site of injury. Objectives: The aim of this study was to determine the effect of the mobilization of autologous bone marrow-derived cells by granulocyte colony-stimulating factor (CSF3) on bleomycin-induced lung injury in mice. Methods: The bone marrow from male green fluorescent protein transgenic (C57Bl/6J) mice was transplanted into irradiated female C57Bl/6J mice. Bleomycin lung injury was induced in these bone marrow-reconstituted mice and unreconstituted C57Bl/6J mice, and some mice were treated with recombinant CSF3. Lung histology, survival, cytokine expression and matrix metalloproteinase (MMP) expression were evaluated to determine the effect of CSF3 after bleomycin-induced lung injury. Results: Histology and flow cytometry analysis showed successful mobilization of bone marrow-derived cells by CSF3 treatment in the recipient lungs. Importantly, CSF3 attenuated bleomycin-induced lung injury and improved survival. Furthermore, CSF3 administration regulated transforming growth factor-β, interferon-γ, MMP9 and tissue inhibitors of MMP1 expression during bleomycin injury. Conclusions: These data demonstrated that the mobilization of bone marrow-derived cells by CSF3 has a protective effect against bleomycin-induced lung injury and fibrosis.
- Published
- 2011
- Full Text
- View/download PDF