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Your search keyword '"Kyvik Ruiz, Adriana"' showing total 3 results
Start Over Author "Kyvik Ruiz, Adriana" Publisher royal society of chemistry (uk)
3 results on '"Kyvik Ruiz, Adriana"'

1. Antibiofilm surfaces based on the immobilization of a novel recombinant antimicrobial multidomain protein using self-assembled monolayers

Author

Centro de Investigación Biomédica en Red Bioingeniería, Biomateriales y Nanomedicina (España), Ministerio de Ciencia, Innovación y Universidades (España), Generalitat de Catalunya, European Commission, Fundació La Marató de TV3, Max Planck Society, Agencia Estatal de Investigación (España), Kyvik Ruiz, Adriana [0000-0002-6385-7162], Roca-Pinilla, Ramon [0000-0002-7393-6200], Mayolo Deloisa, Karla P. [0000-0002-2826-2518], Rodríguez Rodríguez, Xavier [0000-0001-5767-3124], Martinez Miguel, Marc [0000-0001-7722-3657], Martos, Marta [0000-0002-9898-7602], Köber, Mariana [0000-0001-9962-7900], Ventosa, Nora [0000-0002-8008-4974], Veciana, Jaume [0000-0003-1023-9923], Guasch, Judith [0000-0002-3571-4711], Garcia Fruitós, Elena [0000-0001-7498-4864], Arís, Anna [0000-0001-7830-888X], Ratera, Immaculada [0000-0002-1464-9789], Kyvik Ruiz, Adriana, Roca-Pinilla, Ramon, Mayolo Deloisa, Karla P., Rodríguez Rodríguez, Xavier, Martinez Miguel, Marc, Martos, Marta, Köber, Mariana, Ventosa, Nora, Veciana, Jaume, Guasch, Judith, Garcia Fruitós, Elena, Arís, Anna, Ratera, Immaculada, Centro de Investigación Biomédica en Red Bioingeniería, Biomateriales y Nanomedicina (España), Ministerio de Ciencia, Innovación y Universidades (España), Generalitat de Catalunya, European Commission, Fundació La Marató de TV3, Max Planck Society, Agencia Estatal de Investigación (España), Kyvik Ruiz, Adriana [0000-0002-6385-7162], Roca-Pinilla, Ramon [0000-0002-7393-6200], Mayolo Deloisa, Karla P. [0000-0002-2826-2518], Rodríguez Rodríguez, Xavier [0000-0001-5767-3124], Martinez Miguel, Marc [0000-0001-7722-3657], Martos, Marta [0000-0002-9898-7602], Köber, Mariana [0000-0001-9962-7900], Ventosa, Nora [0000-0002-8008-4974], Veciana, Jaume [0000-0003-1023-9923], Guasch, Judith [0000-0002-3571-4711], Garcia Fruitós, Elena [0000-0001-7498-4864], Arís, Anna [0000-0001-7830-888X], Ratera, Immaculada [0000-0002-1464-9789], Kyvik Ruiz, Adriana, Roca-Pinilla, Ramon, Mayolo Deloisa, Karla P., Rodríguez Rodríguez, Xavier, Martinez Miguel, Marc, Martos, Marta, Köber, Mariana, Ventosa, Nora, Veciana, Jaume, Guasch, Judith, Garcia Fruitós, Elena, Arís, Anna, and Ratera, Immaculada

Abstract

The constant increase of microorganisms resistant to antibiotics has been classified as a global health emergency, which is especially challenging when biofilms are formed. Herein, novel biofunctionalized gold surfaces with the antimicrobial multidomain recombinant protein JAMF1, both in the soluble form and nanostructured as nanoparticles, were developed. The interaction between His-tag termination of the protein and a nitriloacetic acid-Ni complex formed on mixed self-assembled monolayers (mixed SAMs) was exploited. The obtained antibiofilm surfaces based on the immobilization of the novel JAMF1 protein using self-assembled monolayers were characterized using a multi-technique approach including: cyclic voltammetry, X-ray photoelectron spectroscopy, atomic force microscopy and fluorescence, proving that the modification and immobilization of JAMF1 were successfully done. The antibiofilm activity against Escherichia coli and carbapenem-resistant Klebsiella pneumoniae showed that the immobilized antimicrobial proteins were able to reduce biofilm formation of both microorganisms. This strategy opens up new possibilities for controlled biomolecule immobilization for fundamental biological studies and biotechnological applications, at the interface of materials science and molecular biology.

Published
2023

2. Stable anchoring of bacteria-based protein nanoparticles for surface enhanced cell guidance

Author

Dirección General de Investigación Científica y Técnica, DGICT (España), Generalitat de Catalunya, Ministerio de Economía y Competitividad (España), European Cooperation in Science and Technology, Fundació La Marató de TV3, European Commission, Max Planck Society, Centro de Investigación Biomédica en Red Bioingeniería, Biomateriales y Nanomedicina (España), Instituto de Salud Carlos III, Institut National de la Recherche Agronomique (France), Martínez Miguel, Marc, Kyvik Ruiz, Adriana, Ernst, Lena M., Martínez Moreno, Albert, Cano Garrido, Olivia, García Fruitós, Elena, Vazquez, Esther, Ventosa, Nora, Guasch, Judith, Veciana, Jaume, Villaverde, Antonio, Ratera, Immaculada, Dirección General de Investigación Científica y Técnica, DGICT (España), Generalitat de Catalunya, Ministerio de Economía y Competitividad (España), European Cooperation in Science and Technology, Fundació La Marató de TV3, European Commission, Max Planck Society, Centro de Investigación Biomédica en Red Bioingeniería, Biomateriales y Nanomedicina (España), Instituto de Salud Carlos III, Institut National de la Recherche Agronomique (France), Martínez Miguel, Marc, Kyvik Ruiz, Adriana, Ernst, Lena M., Martínez Moreno, Albert, Cano Garrido, Olivia, García Fruitós, Elena, Vazquez, Esther, Ventosa, Nora, Guasch, Judith, Veciana, Jaume, Villaverde, Antonio, and Ratera, Immaculada

Abstract

In tissue engineering, biological, physical, and chemical inputs have to be combined to properly mimic cellular environments and successfully build artificial tissues which can be designed to fulfill different biomedical needs such as the shortage of organ donors or the development of in vitro disease models for drug testing. Inclusion body-like protein nanoparticles (pNPs) can simultaneously provide such physical and biochemical stimuli to cells when attached to surfaces. However, this attachment has only been made by physisorption. To provide a stable anchoring, a covalent binding of lactic acid bacteria (LAB) produced pNPs, which lack the innate pyrogenic impurities of Gram-negative bacteria like Escherichia coli, is presented. The reported micropatterns feature a robust nanoscale topography with an unprecedented mechanical stability. In addition, they are denser and more capable of influencing cell morphology and orientation. The increased stability and the absence of pyrogenic impurities represent a step forward towards the translation of this material to a clinical setting.

Published
2020

3. Influence of the donor unit on the rectification ratio in tunnel junctions based on donor– acceptor SAMs using PTM units as acceptors

Author

European Commission, Dirección General de Investigación Científica y Técnica, DGICT (España), Generalitat de Catalunya, Ministerio de Economía y Competitividad (España), Gouvernement fédéral belge, Instituto de Salud Carlos III, Ministry of Education (Singapore), Souto Salom, Manuel, Díez Cabanes, Valentín, Yuan, Li, Kyvik Ruiz, Adriana, Ratera, Immaculada, Nijhuis, Christian A., Corni, Jerome, Veciana, Jaume, European Commission, Dirección General de Investigación Científica y Técnica, DGICT (España), Generalitat de Catalunya, Ministerio de Economía y Competitividad (España), Gouvernement fédéral belge, Instituto de Salud Carlos III, Ministry of Education (Singapore), Souto Salom, Manuel, Díez Cabanes, Valentín, Yuan, Li, Kyvik Ruiz, Adriana, Ratera, Immaculada, Nijhuis, Christian A., Corni, Jerome, and Veciana, Jaume

Abstract

Dyads formed by an electron donor unit (D) covalently linked to an electron acceptor (A) by an organic bridge are promising materials as molecular rectifiers. Very recently, we have reported the charge transport measurements across self-assembled monolayers (SAMs) of two D–A systems consisting of the ferrocene (Fc) electron-donor linked to a polychlorotriphenylmethane (PTM) electron-acceptor in its non-radical (SAM 1) and radical (SAM 2) forms. Interestingly, we observed that the non-radical SAM 1 showed rectification behavior of 2 orders of magnitude higher than its radical analogue dyad 2. In order to study the influence of the donor unit on the transport properties, we report herein the synthesis and characterization of two new D–A SAMs in which the electron-donor Fc unit is replaced by a tetrathiafulvalene (TTF) moiety linked to the PTM unit in its non-radical (SAM 3) and radical (SAM 4) forms. The observed decrease in the rectification ratio and increased current density for TTF-PTM based SAMs 3 and 4 in comparison to Fc-PTM based SAMs 1 and 2 are explained, supported by theoretical calculations, by significant changes in the electronic and supramolecular structures.

Published
2018

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