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Your search keyword '"Kris M. Weigel"' showing total 2 results
Start Over Author "Kris M. Weigel" Publisher royal society of chemistry (rsc)
2 results on '"Kris M. Weigel"'

1. Integrated nucleic acid testing system to enable TB diagnosis in peripheral settings

Author

Pranav Kumar, Hsiang-Wei Lu, Gerard A. Cangelosi, Ryan P. Talbot, David S. Boyle, Masahiro Narita, Rama Murthy Sakamuri, Robert Doebler, Kris M. Weigel, Keith Herrington, Tanya M. Ferguson, Felicia K. Nguyen, and Angelika Niemz

Subjects
Tuberculosis, Biomedical Engineering, Bioengineering, Sensitivity and Specificity, Biochemistry, Article, Mycobacterium tuberculosis, Cartridge, Nucleic Acids, medicine, Humans, Detection limit, Chromatography, biology, business.industry, Sputum, General Chemistry, Amplicon, medicine.disease, biology.organism_classification, DNA extraction, Nucleic acid, medicine.symptom, business, Nucleic Acid Amplification Techniques
Abstract

To facilitate treatment and limit transmission of tuberculosis (TB), new methods are needed to enable rapid and affordable diagnosis of the disease in high-burden low-resource settings. We have developed a prototype integrated nucleic acid testing device to detect Mycobacterium tuberculosis (M.tb) in sputum. The device consists of a disposable cartridge and compact, inexpensive instrument that automates pathogen lysis, nucleic acid extraction, isothermal DNA amplification and lateral flow detection. A liquefied and disinfected sputum sample is manually injected into the cartridge, and all other steps are automated, with a result provided in

Published
2020
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2. Label-free electrochemical detection of an Entamoeba histolytica antigen using cell-free yeast-scFv probes

Author

Matt Trau, Yadveer S. Grewal, Sean A. Gray, Gerard A. Cangelosi, Kris M. Weigel, and Muhammad J. A. Shiddiky

Subjects
Antigens, Protozoan, Saccharomyces cerevisiae, Article, Catalysis, law.invention, Electron Transport, Entamoeba histolytica, Antigen, law, Materials Chemistry, medicine, Humans, Ferricyanides, Electrodes, Pathogen, Immunoassay, biology, medicine.diagnostic_test, Chemistry, Metals and Alloys, Electrochemical Techniques, General Chemistry, biology.organism_classification, Molecular biology, Recombinant Proteins, Yeast, Surfaces, Coatings and Films, Electronic, Optical and Magnetic Materials, Ceramics and Composites, biology.protein, Recombinant DNA, Gold, Antibody, Single-Chain Antibodies
Abstract

Inexpensive, simple and quick detection of pathogen antigens in human samples is a key global health objective. Limiting factors include the cost and complexity of diagnostic tests that utilize antibody probes. Herein, we present a method for label-free electrochemical detection of a protein from the enteric pathogen Entamoeba histolytica using cell-free yeast-embedded antibody-like fragments (yeast-scFv) as novel affinity reagents.

Published
2013
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