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An efficient electrochemical trifluoromethylation of coumarins using CF3SO2NHNHBoc as the source of the trifluoromethyl group was developed.

Filtration membranes are widely applied in medical fields. However, these membranes are challenged by bacterial contamination in hospitals, which increases the risk of nosocomial infections. Thus, it is significant to develop antibacterial filtration membranes. In this work, an oxidated dextran (ODex)-based antibacterial coating was designed and constructed on microfiltration (MF) membranes and melt-blown fabrics. Polyhexamethylene guanidine (PHMG) was synthesized as an antibacterial agent, and was fixed by ODex onto filtration membranes. The functionalized MF membranes increased the filtration efficiency for E. coli from 20.9% to 99.9%, and improved the absorption ratio for endotoxin by 59.1%, while the water flow rate still remained as high as 5255 L (h m2)−1. Furthermore, the trapped bacteria were inactivated by the antibacterial coating. For the melt-blown fabrics, the aerosol filtration efficiency was increased from 74.6% to 81.0%, and the antibacterial efficiency was promoted to 92.0%. The present work developed a facile and universal antibacterial functionalization strategy for filtration membranes, which provided a new method for the design and development of various novel antibacterial filtration materials in the medical field.

Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/CRISPR-associated protein 9 (Cas9) gene editing technology is a promising approach for cancer therapy, and its application practice urgently requires a safe and effective gene carrier. In this work, we focus on the design of a phenylboronic acid (PBA)-functionalized, disulfide bonded branched polyaminoglycoside (SS-HPT-P) as a robust delivery vector of the CRISPR-Cas9 system. SS-HPT-P showed great tumor-targeting performance, reduction-responsive degradability, and gene transfection ability. The typical pCas9-surv (one CRISPR-Cas9 plasmid that targets and knocks out the survivin gene) delivery mediated by SS-HPT-P exhibited gene editing performance in the A549 cell line, confirming the feasibility of SS-HPT-P to effectively deliver the CRISPR-Cas9 system. SS-HPT-P/pCas9-surv could effectively inhibit the proliferation of tumor cells both in vitro and in vivo, suggesting the potential of PBA-functionalized nanocarriers for cancer gene therapy. The present work provides a promising approach for the treatment of malignant tumors.