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Serum alanine aminotransferase (ALT) is the most sensitive indicator of liver function; therefore, in clinical practice, its detection has diagnostic significance. However, hepatotoxicity monitoring is constrained in resource-limited areas due to the high cost and expensive equipment. This article describes a low-cost, compact blood enzyme analyzer (BEA) for point-of-care (PoC) liver function testing that uses reflection photometry to quantitate the colorimetric enzyme assay results. Moreover, the analyzer incorporates rapid and constant 37 °C temperature control to ensure that human serum enzymes remain active. The BEA was used to evaluate ALT in 50 whole blood samples using PoC photochemical test strips. The test results showed a linear correlation coefficient of 0.9749 compared with a clinical-specific biochemical analyzer and coefficients of variation (CV)% less than 5%. It has a low detection limit of 3.62 U L−1 and a wide detection range of 4–480 U L−1. In addition, the BEA enables smartphone access to the Internet of Medical Things (IoMT) via Bluetooth to facilitate active chronic disease management or remote diagnosis in PoC settings. Therefore, the BEA is a promising system for health management using the IoMT. [ABSTRACT FROM AUTHOR]

Coulter counters are ubiquitous in everyday life; however, with reduced orifice size shrinkage, there is an increased risk of clogging. Herein, a 3D microfluidic printing-based single-use kit is presented for analyzing biological samples and performing accurate Coulter cell count analysis (e.g., white blood cells in the blood). The gem hole eliminates the traditional design concept of integration inside the detection instrument, innovatively causing it to be independent from the analysis instrument. Further, integrating the hole in the analysis box enables the design of a separate detection module. The analysis box is disposable, convenient, and hygienic; avoids cross-infection; solves the problem of clogging of tiny holes from a new perspective; and no longer requires uneconomical and inconvenient methods, such as flushing, cauterization, and fluid focusing, through solid water flow. Further development of the newly designed 3D printing analysis box can enable its extensive use in POCT (point-of-care) detection scenarios. Moreover, through mass production, the issue of cost will be eliminated. [ABSTRACT FROM AUTHOR]