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A mono-pyrene substituted thiacalix[4]arene chemosensor (TCA-Py) was successfully synthesized in satisfactory yield. Fluorescence analysis revealed that TCA-Py exhibited a high recognition selectivity toward the Al(ClO4)3 molecule due to the synergy between the Al3+ cation and ClO4− anion. This unique ability to recognise an entire inorganic molecule broadens the field of molecular recognition. [ABSTRACT FROM AUTHOR]

MicroRNAs (miRNAs) have attracted extensive interest as promising biomarkers for the profiling of diseases. However, quantitative measurement of miRNAs presents a significant challenge in biochemical studies. In this work, we developed an innovative optofluidic platform to perform a rapid, simple, quantitative and high-specificity miRNA assay using the Förster resonance energy transfer (FRET) principle. A novel three-way junction FRET probe was proposed to enable rapid and enzyme-free miRNA detection. Using this platform, we performed one-step, amplification-free miRNA detection with simple device operation and achieved miRNA identification at a low concentration. The detection system could achieve high specificity for discrimination of three-base mismatches, and the sample volume was significantly reduced, favorable for low-level miRNA detection in material-limited samples. The establishment of a compact, low-cost, highly sensitive and selective miRNA analysis platform provides a valuable tool for point-of-care diagnosis. [ABSTRACT FROM AUTHOR]

A mono-pyrene substituted thiacalix[4]arene chemosensor (TCA-Py) was successfully synthesized in satisfactory yield. Fluorescence analysis revealed that TCA-Py exhibited a high recognition selectivity toward the Al(ClO 4 ) 3 molecule due to the synergy between the Al 3+ cation and ClO 4 - anion. This unique ability to recognise an entire inorganic molecule broadens the field of molecular recognition.

A tripodal fluorescent probe L1 armed with Rhodamine B and 1-naphthaleneisothiocyanates was prepared in high yield. A study of the recognition properties revealed that probe L1 exhibited high sensitivity and selectivity towards Al 3+ through a "FRET" fluorescence response and colorimetric response with low detection limits of the order of 10 -8 M. Meanwhile, probe L1 also possessed high recognition ability for I - through fluorescence decay, which given there are comparatively few selective fluorescent probes for I - , is significant. Furthermore, the complexation mechanisms were fully investigated by spectral titrations, 1 H NMR spectroscopic titrations and mass spectrometry. The utility of probe L1 as a biosensor in living cells (PC3 cells) towards Al 3+ ions has also been demonstrated.