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Neurological disorders are closely linked to the alterations in cell membrane permeability (CMP) and mitochondrial membrane potential (MMP). Changes in CMP and MMP may lead to damage and death of nerve cells, thus triggering the onset and progression of neurological diseases. Therefore, monitoring the changes of these two physiological parameters not only benefits the accurate assessment of nerve cell health status, but also enables providing key information for the diagnosis and treatment of neurological diseases. However, the simultaneous monitoring of these two cellular physiological parameters is still challenging. Herein, we design and synthesize two quinolinium-carbazole-derivated fluorescent probes (OQ and PQ). As isomers, the only difference in their chemical structures is the linking position of the carbazole unit in quinoline rings. Strikingly, such a subtle difference endows OQ and PQ with significantly different organelle-staining behaviors. PQ mainly targets at the nucleus, OQ can simultaneously stain cell membranes and mitochondria in normal cells, and performs CMP and MMP-dependent translocation from the cell membrane to mitochondria then to the nucleus, thus holding great promise as an intracellular translocation probe to image the changes of CMP and MMP. After unraveling the intrinsic mechanism of their different translocation abilities by combining experiments with molecular dynamics simulations and density functional theory calculations, we successfully used OQ to monitor the continuous changes of CMP and MMP in three neurological disease-related cell models, including oxidative stress-damaged, Parkinson's disease, and virus-infected ones. Besides providing a validated imaging tool for monitoring cellular physiological parameters, this work paves a promising route for designing intracellular translocation probes to analyze cellular physiological parameters associated with various diseases. [ABSTRACT FROM AUTHOR]

With the aid of the single-virus tracking technique and the quantum dot-labeling strategy, the internalization of the pseudorabies virus (PrV) has been visualized in real time. The results demonstrate that macropinocytosis can be considered as a major pathway for PrV entering HeLa cells, which facilitates the development of therapeutics for virus-triggered diseases. [ABSTRACT FROM AUTHOR]

Droplet-based microfluidic chips have shown great advantages in fields of chemical and biological researches. Based on the different wettabilities of water, IL (ionic liquid, 1-butyl-3-methylimidazolium hexafluorophosphate) and soybean oil in hydrophobic channels, three kinds of droplet pairs (including an alternating water–IL droplet chain, connected water–IL droplet pairs and separated water–IL droplet pairs) were generated for the first time in soybean oil on microfluidic chips containing double flow-focusing regions. The influences of fluid flow rate and channel geometry on droplet pair formation were carefully studied. [ABSTRACT FROM AUTHOR]

Hydrogels having strong fluorescence were fabricated successfully, for the first time, from cellulose and quantum dots (QDs) in a NaOH/urea aqueous system viaa mild chemical cross-linking process. The CdSe/ZnS nanoparticles were embedded firmly in the cellulose matrices, as a result of strong interactions between the CdSe/ZnS nanoparticles and cellulose after hydrolysis of the QDs ligands. The results from FTIR spectra, transmission electron microscopy, scanning electron microscopy and photoluminescence (PL) spectra revealed that the cellulose networks in the hydrogels played an important role in the protection of the CdSe/ZnS structure and preservation of the quantum dots characteristics. The cellulose–QDs hydrogels exhibited strong PL emission and nearly pure color from green to red, depending on the size of the QDs. Moreover, the hybrid hydrogels possessed a good transparence and compressive strength. This work provides a new pathway for the construction of safe and biocompatible biopolymer–QDs hydrogels with a high efficiency of PL emission. [ABSTRACT FROM AUTHOR]

We have developed a convenient strategy for preparing color-tunable fluorescent-magnetic core/shell multifunctional nanocrystals, which exhibit excellent photoluminescence (PL) properties (fluorescing tunably from 550 nm to 630 nm by modifying the shell thickness) and ferromagnetic material properties (a magnetization of 4.4 emu g-1 and a coercivity of 95 Oe). [ABSTRACT FROM AUTHOR]

A simple and facile method was developed to prepare fluorescent carbon nanocrystals (CNCs) with low cytotoxicity and no photobleaching, by electrooxidation of graphite in aqueous solution. [ABSTRACT FROM AUTHOR]

We present a method that allows patterning cells and shear flow conditions for endothelial cell based assays. This method is novel in combining (1) cell culture on the surface of a substrate both topographically and chemically patterned; (2) multi-shear flow assays after covering the cell substrate with a microfluidic cover plate containing microchannels of different channel widths, and (3) conventional immunostaining assays after removal of the cover plate. This method has the advantage of performing cell cultures and immunoassays in standard cell biology environments with open access, facilitating the formation of confluent cell layers and the observation of cell responses to shear-flow and drug stimulations. To obtain multi-shear stress conditions, a single channel with stepwise increasing channel widths was patterned on the surfaces of both the substrate and the microfluidic cover plate. As results, we observed excellent viability of endothelial cells in the whole range of applied shear stresses (0–25 dyn cm−2) and shear stress dependent cytoskeleton remoulding, activation of von Willebrand factor (vWF), and re-organisation of angiogenesis factors such as tetra peptide acetyl-Ser-Asp-Lys-Pro (AcSDKP) of endothelial cells. To validate this approach for drug analysis, we also studied drug effects under shear stress conditions. Our results indicate that the drug effect of combretastatin A-4, an anti-tumour vascular targeting drug, could be significantly enhanced under shear flow conditions. [ABSTRACT FROM AUTHOR]

The New Journal of Chemistry is a publication that focuses on chemistry research. The document provides a list of articles included in the current issue, covering topics such as fungicides, fuel cells, and photocatalytic materials. The authors of these articles come from different institutions and countries. The document also includes information for authors on how to submit their work for publication. [Extracted from the article]

The New Journal of Chemistry, published by the Royal Society of Chemistry and the Centre National de la Recherche Scientifique, features a collection of papers covering various topics in chemistry. The papers focus on specific research studies and provide detailed information on methods and results. Topics include catalysis, nanomaterials, photocatalysis, and electrochemistry. Written by authors from different institutions, these papers offer a comprehensive overview of recent research in the field of chemistry. [Extracted from the article]

The New Journal of Chemistry is a publication that focuses on chemistry research. It includes articles on various topics such as the preparation of silver nanoparticles, the study of physical properties of inclusion complexes, the characterization of modified methylaluminoxane, and the synthesis of compounds for different applications. The document provides contact information for the journal and details about subscriptions. It also includes a correction for a previous paper. [Extracted from the article]

The New Journal of Chemistry, published by the Royal Society of Chemistry, features articles on a wide range of topics in chemistry. The current issue covers subjects such as nanosized metals, liquid catalyzed growth of graphene, and photocatalytic CO2 reduction. The journal aims to provide innovative and cutting-edge reports that have a strong chemical component and broad appeal. This document is a compilation of research papers from the journal, covering topics such as electrocatalysts, nanocomposites, halogenation reactions, supramolecular assembly, and more. The papers explore various applications, including agriculture and waste digestion. [Extracted from the article]

The New Journal of Chemistry is a publication by the Royal Society of Chemistry that focuses on publishing innovative and high-quality research in the field of chemistry. The contents list provides information about the purpose of the journal and includes articles on topics such as catalytic oxidation of carbon monoxide and synthesis of spirotriazolines and spirooxadiazolines. The journal covers a wide range of topics including the synthesis of nanoparticles, catalytic reactions, electrochemical reduction, and drug discovery. The papers offer insights into scientific phenomena and potential applications in fields such as energy storage, wastewater treatment, and medicine. [Extracted from the article]

The document is a contents list for the New Journal of Chemistry, a publication that focuses on scientific advancements and applications in fields such as nanotechnology, chemistry, and materials science. It includes a list of papers published in 2023, covering topics such as light amplification, synthesis of selenophenes, selective catalytic reduction, catalytic oxidation of pollutants, glucose sensing, sensing for Hg2+, drug dissociation, catalytic degradation of polyester, and coordination reactions. The document also provides information about the journal's purpose, the organizations involved in its publication, and the editorial staff and advisory board. [Extracted from the article]

The document is a contents list of the New Journal of Chemistry, a publication that focuses on various topics in the field of chemistry. It includes a list of articles in the current issue, along with the names of the authors and a brief description of each article. The journal covers a wide range of subjects, such as catalyst synthesis, drug discovery, nanotechnology, and chemical reactions. The document also provides information about the editorial staff and contact details for submitting articles or making inquiries. It concludes with details about subscriptions and copyright. This document serves as a valuable resource for researchers interested in accessing the latest research in chemistry. [Extracted from the article]

The document is a contents list for the New Journal of Chemistry, a publication by The Royal Society of Chemistry and the Centre National de la Recherche Scientifique. It includes articles on topics such as compound synthesis, catalytic activity, drug delivery, and energy storage. The authors and their affiliations are listed, and there is information for authors on how to submit material for publication. This document provides valuable information for researchers in these fields. [Extracted from the article]

The New Journal of Chemistry is a publication that features research papers on various topics in chemistry. The current issue includes articles on the synthesis of compounds, the development of catalysts, and the investigation of chemical reactions. Each paper presents its own findings and conclusions based on the research conducted. The document also provides information for authors on how to submit their own material for publication. [Extracted from the article]

The New Journal of Chemistry is a publication that features research papers on various topics related to chemistry. It is a collaboration between The Royal Society of Chemistry and the Centre National de la Recherche Scientifique. The current issue includes articles on the stabilization of emulsion gels, antioxidant activity of lignans, electrostatic potential in hydrogen bond formation, and the synthesis of compounds for different applications. Each paper provides detailed information on the research conducted and the findings obtained. The document also provides information for authors on how to submit their own research for publication. [Extracted from the article]

Influenza A virus (IAV) hijacks the endocytosis of host cells in the early stages of infection. It is known that microfilaments and microtubules play a vital role in viral infections. However, the potential involvement of intermediate filaments (IFs) and their mechanism in IAV infection are unclear. In this study, we combined quantum dot-based single-virus tracking (QSVT) with conventional biological methods to quantitatively analyze the effects of vimentin IFs on the early stages of IAV infection. The results showed that the knockdown of vimentin did not affect the internalization and motion dynamics of IAV but impacted the transport of IAV to late endosomes. Statistical analysis revealed that the knockdown of vimentin caused more than 20% IAV to remain in the early endosomes after rapid movement than in normal cells and reduced the efficiency of subsequent membrane fusion and genome release. These results suggested that vimentin facilitates IAV infection by participating in the maturation of endosomes. Our study expands the understanding of the mechanism of endocytosis of IAV and fills the gap in the knowledge on vimentin IFs. [ABSTRACT FROM AUTHOR]