478,817 results
Search Results
2. A paper-based dual functional biosensor for safe and user-friendly point-of-care urine analysis.
- Author
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Li Y, Kong Y, Hu Y, Li Y, Asrosa R, Zhang W, Deka Boruah B, Yetisen AK, Davenport A, Lee TC, and Li B
- Subjects
- Humans, Hydrogen-Ion Concentration, Gold chemistry, Glucose analysis, Urinalysis instrumentation, Glucose Oxidase chemistry, Glucose Oxidase metabolism, Electrochemical Techniques, Metal Nanoparticles chemistry, Graphite chemistry, Biomarkers urine, Biosensing Techniques, Paper, Point-of-Care Systems
- Abstract
Safe, accurate, and reliable analysis of urinary biomarkers is clinically important for early detection and monitoring of the progression of chronic kidney disease (CKD), as it has become one of the world's most prevalent non-communicable diseases. However, current technologies for measuring urinary biomarkers are either time-consuming and limited to well-equipped hospitals or lack the necessary sensitivity for quantitative analysis and post a health risk to frontline practitioners. Here we report a robust paper-based dual functional biosensor, which is integrated with the clinical urine sampling vial, for the simultaneous and quantitative analysis of pH and glucose in urine. The pH sensor was fabricated by electrochemically depositing IrOx onto a paper substrate using optimised parameters, which enabled an ultrahigh sensitivity of 71.58 mV pH
-1 . Glucose oxidase (GOx) was used in combination with an electrochemically deposited Prussian blue layer for the detection of glucose, and its performance was enhanced by gold nanoparticles (AuNPs), chitosan, and graphite composites, achieving a sensitivity of 1.5 μA mM-1 . This dual function biosensor was validated using clinical urine samples, where a correlation coefficient of 0.96 for pH and 0.98 for glucose detection was achieved with commercial methods as references. More importantly, the urine sampling vial was kept sealed throughout the sample-to-result process, which minimised the health risk to frontline practitioners and simplified the diagnostic procedures. This diagnostic platform, therefore, holds high promise as a rapid, accurate, safe, and user-friendly point-of-care (POC) technology for the analysis of urinary biomarkers in frontline clinical settings.- Published
- 2024
- Full Text
- View/download PDF
3. Chitosan-adhered graphene/nano iron tetroxide carbon paper electrode for the detection of hexavalent chromium.
- Author
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Liangyi Tian, Ting Huang, Yilei Xiang, Yichun Bi, Zihan Yu, Jihuan Xie, Jingping Qiu, Lemin Chen, and Linbin Jiang
- Subjects
- *
CARBON electrodes , *CARBON paper , *HEXAVALENT chromium , *MAGNETITE , *IRON , *GRAPHENE , *CHROMIUM compounds - Abstract
This report presents an investigation into the use of carbon paper electrodes prepared from chitosan-adhered graphene magnetite nanoparticles for the analysis of Cr(VI). The preparation and storage of carbon paper electrodes is a simple process, and these electrodes are easier to replace than electrodes modified by more conventional methods. The electrochemical detection of Cr(VI) using square wave adsorption cathodic dissolution voltammetry (SWAdCSV) with high selectivity and sensitivity, as well as the optimum conditions for the preparation of the electrodes and the electrode parameters affecting the SWAdCSV signal, were the main points of focus of the investigation. Cr(VI) was detected linearly in the range of 4-40 μg L-1 with a detection limit of 2.84 μg L-1. The electrode output precision, calculated as %RSD, was 7.16% (n = 6), and this was the measurement used for the detection of Cr(VI) in standard and test samples with recoveries between 89% and 114%. The results were consistent with those obtained using the standard UV-Vis spectrophotometric method with a paired t-test at 95% confidence level. [ABSTRACT FROM AUTHOR]
- Published
- 2024
- Full Text
- View/download PDF
4. Used tissue paper as a 3D substrate for non-enzyme glucose sensors.
- Author
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Zhiyu Chen, Lei Li, Xuanyu Xiao, Yuxin Zhang, Jieyu Zhang, Qing Jiang, Xuefeng Hu, and Yunbing Wang
- Subjects
- *
GLUCOSE analysis , *PAPER recycling , *OXIDATION of glucose , *RECYCLED paper , *DETECTORS , *DENSITY functional theory - Abstract
Non-enzymatic electrochemical blood glucose sensors often suffer from issues such as requiring an alkaline environment, limited monitoring range, and poor anti-interference properties. Carbon substrates have been demonstrated to improve the performance of non-enzymatic sensors, but complex and energy-consuming manufacturing processes restrict their use. Herein, a simple and green approach for the preparation of 3D porous Au/Au-Pt networks (PAAPNs) is proposed using tissue paper for recycling as the nanomaterial substrate to deposit Au-Pt bimetallic nanoparticles. The unique structure of the PAAPNs sensor allows for low-potential operation (-1.0 V) with a wide monitoring range (-0.25 to 36 mM) in a neutral environment. Moreover, the sensor exhibited excellent selectivity (<10% response of interference) even without the use of an anti-interference outer membrane. Further investigation using density functional theory (DFT) simulation revealed the synergistic effect between Au and Pt in promoting glucose oxidation. Overall, this work provides a simple and low-carbon footprint method for creating carbon substrates from tissue paper for recycling, offering new opportunities for fabricating novel value-added nanomaterials for medical sensing applications. [ABSTRACT FROM AUTHOR]
- Published
- 2024
- Full Text
- View/download PDF
5. Paper spray mass spectrometry combined with machine learning as a rapid diagnostic for chronic kidney disease.
- Author
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Pereira I, Sboto JNS, Robinson JL, and Gill CG
- Subjects
- Humans, Paper, Albuminuria diagnosis, Albuminuria urine, Rapid Diagnostic Tests, Renal Insufficiency, Chronic diagnosis, Renal Insufficiency, Chronic urine, Machine Learning, Mass Spectrometry methods
- Abstract
A new analytical method for chronic kidney disease (CKD) detection utilizing paper spray mass spectrometry (PS-MS) combined with machine learning is presented. The analytical protocol is rapid and simple, based on metabolic profile alterations in urine. Anonymized raw urine samples were deposited (10 μL each) onto pointed PS-MS sample strips. Without waiting for the sample to dry, 75 μL of acetonitrile and high voltage were applied to the strips, using high resolution mass spectrometry measurement (15 s per sample) with polarity switching to detect a wide range of metabolites. Random forest machine learning was used to classify the resulting data. The diagnostic performance for the potential diagnosis of CKD was evaluated for accuracy, sensitivity, and specificity, achieving results >96% for the training data and >91% for validation and test data sets. Metabolites selected by the classification model as up- or down-regulated in healthy or CKD samples were tentatively identified and in agreement with previously reported literature. The potential utilization of this approach to discriminate albuminuria categories (normo, micro, and macroalbuminuria) was also demonstrated. This study indicates that PS-MS combined with machine learning has the potential to be used as a rapid and simple diagnostic tool for CKD.
- Published
- 2024
- Full Text
- View/download PDF
6. A paper-based ratiometric fluorescence sensor based on carbon dots modified with Eu3+ for the selective detection of tetracycline in seafood aquaculture water.
- Author
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Zhang, Jialu, Chen, Yuanyuan, Qi, Ji, Miao, Qinglan, Deng, Dongmei, He, Haibo, Yan, Xiaoxia, and Luo, Liqiang
- Subjects
FLUORESCENCE ,SEAFOOD ,TETRACYCLINE ,TETRACYCLINES ,AQUACULTURE ,DETECTORS - Abstract
Paper-based ratiometric fluorescence sensors are normally prepared using two or more types of fluorescent materials on a paper chip for simple, low-cost and fast detection. However, the choice of multi-step and one-step modifications on the paper chip affects the analytical performance. Herein, a novel paper-based dual-emission ratiometric fluorescence sensor was designed for the selective detection of tetracycline (TC). Carbon dots (CDs) modified with Eu
3+ were combined with a sealed paper-based microfluidic chip by two methods: one-step grafting of CDs-Eu3+ on paper and step-by-step grafting of CDs and Eu3+ on paper. The analytical performance was studied and optimized respectively. The red fluorescence of Eu3+ at 450 nm is enhanced and the blue fluorescence of CDs at 617 nm is quenched by energy transfer in the presence of TC. Under optimal conditions, TC is selectively determined in the linear range from 0.1 μM to 100 μM with a detection limit of 0.03 μM by the step-by-step grafting method. In addition, the sealed paper chip could effectively prevent pollution and volatilization from the reagent. This technique has been used to analyze TC in seafood aquaculture water with satisfactory results. [ABSTRACT FROM AUTHOR]- Published
- 2024
- Full Text
- View/download PDF
7. Office paper and laser printing: a versatile and affordable approach for fabricating paper-based analytical devices with multimodal detection capabilities.
- Author
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Sousa, Lucas R., Guinati, Barbara G. S., Maciel, Lanaia I. L., Baldo, Thaisa A., Duarte, Lucas C., Takeuchi, Regina M., Faria, Ronaldo C., Vaz, Boniek G., Paixão, Thiago R. L. C., and Coltro, Wendell K. T.
- Subjects
LASER printing ,PRINTMAKING ,PROSTATE-specific antigen ,ELECTROCHEMICAL analysis ,ELECTROSPRAY ionization mass spectrometry ,IRON ,PHOTOVOLTAIC power systems - Abstract
Multiple protocols have been reported to fabricate paper-based analytical devices (PADs). However, some of these techniques must be revised because of the instrumentation required. This paper describes a versatile and globally affordable method to fabricate PADs using office paper as a substrate and a laser printing technique to define hydrophobic barriers on paper surfaces. To demonstrate the feasibility of the alternatives proposed in this study, the fabrication of devices for three types of detection commonly associated with using PADs was demonstrated: colorimetric detection, electrochemical detection, and mass spectrometry associated with a paper-spray ionization (PSI-MS) technique. Besides that, an evaluation of the type of paper used and chemical modifications required on the substrate surface are also presented in this report. Overall, the developed protocol was suitable for using office paper as a substrate, and the laser printing technique as an efficient fabrication method when using this substrate is accessible at a resource-limited point-of-need. Target analytes were used as a proof of concept for these detection techniques. Colorimetric detection was carried out for acetaminophen, iron, nitrate, and nitrite with limits of detection of 0.04 μg, 4.5 mg mL
−1 , 2.7 μmol L−1 , and 6.8 μmol L−1 , respectively. A limit of detection of 0.048 fg mL−1 was obtained for the electrochemical analysis of prostate-specific antigen. Colorimetric and electrochemical devices revealed satisfactory performance when office paper with a grammage of 90 g m−2 was employed. Methyldopa analysis was also carried out using PSI-MS, which showed a good response in the same paper weight and behavior compared to chromatographic paper. [ABSTRACT FROM AUTHOR]- Published
- 2024
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- View/download PDF
8. A wash-free, elution-free and low protein adsorption paper-based material for nucleic acid extraction.
- Author
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Tang, Ruihua, Yan, Xueyan, Li, Min, Du, Aoqi, Yang, Hui, Yin, Huancai, and Xie, Mingyue
- Published
- 2023
- Full Text
- View/download PDF
9. A supramolecular polymer network constructed using a pillararene-based multi-functional monomer and its application as a rewritable fluorescent paper.
- Author
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Liang, Bicong, Xia, Danyu, Cheng, Yujie, Zheng, Qiang, and Wang, Pi
- Subjects
- *
ELECTRONIC paper , *SUPRAMOLECULAR polymers , *FLUORESCENT polymers , *MONOMERS , *POLYMER networks - Abstract
A simple and mild stimulus-responsive fluorescent supramolecular polymer network was constructed from a pillararene-based multi-functional monomer through multiple noncovalent interactions and used as a rewritable paper. [ABSTRACT FROM AUTHOR]
- Published
- 2023
- Full Text
- View/download PDF
10. Rapid fabrication of hydrophobic/hydrophilic patterns on paper substrates for paper spray mass spectrometry.
- Author
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Arias, Austin, Windham, Peyton E., Cheyne, Natalie A., and Gilliland, William M.
- Subjects
EFAVIRENZ ,MASS spectrometry ,ENERGY dispersive X-ray spectroscopy ,HYDROPHOBIC surfaces ,CONTACT angle ,DETECTION limit ,OXYGEN masks - Abstract
A simple, rapid chemical coating and patterning method was developed and optimized for paper-based substrates for use in paper spray mass spectrometry (PS-MS). A variety of chlorosilanes were explored for coating paper substrates, and their effectiveness in forming hydrophobic surfaces was characterized via contact angle goniometry, scanning electron microscopy, and energy dispersive X-ray spectroscopy. Trichloromethylsilane was selected as the primary coating agent because of the short time required to produce a hydrophobic surface (contact angle > 130°), as well as the ease of patterning. Patterning was performed using 3D-printed masks and an oxygen/plasma cleaner. Optimal mask thickness and oxygen/plasma cleaning parameters were determined to produce channels varying from 0.5 to 2.5 mm in width. The effectiveness of the patterned substrates for PS-MS was determined via analysis of four antiretrovirals: emtricitabine, lamivudine, efavirenz, and dolutegravir. Calibration curves were made for each antiretroviral at varying channel widths, and the limits of detection and limits of quantification for each drug were determined. These results show that this patterning method results in an average 7.2-fold improvement in sensitivity and an average 190-fold improvement in limits of detection over uncoated paper substrates in a neat matrix. In a proof-of-concept experiment, calibration curves were generated for each antiretroviral in urine. A patterned paper substrate with a 2-mm channel resulted in an average 7.4-fold improvement in sensitivity and an average 18-fold improvement in limits of detection over uncoated paper substrates. [ABSTRACT FROM AUTHOR]
- Published
- 2023
- Full Text
- View/download PDF
11. Fluorescence sensor using porous host molecules deposited on filter paper with vapochromic and mechanochromic properties.
- Author
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Umezane, Sota, Fukutomi, Satoshi, Ono, Toshikazu, Hisaeda, Yoshio, Nishimura, Tomoki, Kawasaki, Riku, and Ikeda, Atsushi
- Subjects
FILTER paper ,FLUORESCENCE ,IRRADIATION ,ABSORBED dose ,MOLECULES ,DETECTORS - Abstract
Naphthalenediimide derivative-absorbed papers, which were prepared by dropping chloroform solution, showed fluorescence under photoirradiation after exposure to vapors of small aromatic guests. The fluorescence intensity and maximum wavelength depended on the type of guest aromatic molecules, similar to the crystal. The naphthalenediimide derivative-absorbed papers showed vapochromic and mechanochromic properties. [ABSTRACT FROM AUTHOR]
- Published
- 2023
- Full Text
- View/download PDF
12. Generating signals at converging liquid fronts to create line-format readouts of soluble assay products in three-dimensional paper-based devices.
- Author
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Abdullah IH, Wilson DJ, Mora AC, Parker RW, and Mace CR
- Subjects
- Acetylcholinesterase, Ferric Compounds, Paper, Biological Assay, Lab-On-A-Chip Devices, Microfluidic Analytical Techniques
- Abstract
The correct interpretation of the result from a point-of-care device is crucial for an accurate and rapid diagnosis to guide subsequent treatment. Lateral flow tests (LFTs) use a well-established format that was designed to simplify the user experience. However, the LFT device architecture is inherently limited to detecting analytes that can be captured by molecular recognition. Microfluidic paper-based analytical devices (μPADs), like LFTs, have the potential to be used in diagnostic applications at the point of care. However, μPADs have not gained significant traction outside of academic laboratories, in part, because they have often demonstrated a lack of homogeneous shape or color in signal outputs, which consequently can lead to inaccurate interpretation of results by users. Here, we demonstrate a new class of μPADs that form colorimetric signals at the interfaces of converging liquid fronts ( i.e. , lines) to control where colorimetric signals are formed without relying on capture techniques. We demonstrate our approach by developing assays for three classes of analytes-an ion, an enzyme, and a small molecule-to measure using iron(III), acetylcholinesterase, and lactate, respectively. Additionally, we show these devices have the potential to support multiplexed assays by generating multiple lines in a common readout zone. These results highlight the ability of this new paper-based device architecture to aid the interpretation of assays that create soluble products by using flow to constrain those colorimetric products in a familiar, line-format output.
- Published
- 2023
- Full Text
- View/download PDF
13. Equipment-free determination of ascorbic acid based on the UV-induced oxidation of 3,3′,5,5′-tetramethylbenzidine in a paper-based analysis device.
- Author
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Liu, Xiaoxia, Hou, Wenya, Zhao, Jinzhong, Zhang, Lili, Li, Anping, and Ma, Ruiyan
- Subjects
VITAMIN C ,ANALYSIS of colors ,OXIDATION ,FILTER paper ,HYDROXYL group ,BLUE - Abstract
A simple, portable and cost-effective paper-based analysis device is developed for quantitative determination of ascorbic acid (AA) based on the UV-induced oxidation of 3,3′,5,5′-tetramethylbenzidine (TMB). The method does not involve the introduction of any enzyme or external oxidants. More importantly, large equipment is not needed and only a 20 μL sample is needed for each analysis which only takes 8 min. The filter paper is pre-treated with TMB and it turns blue upon UV irradiation because TMB is a self-photosensitizer. The phenol hydroxyl groups of AA can inhibit photo-triggered oxidation resulting in a lighter blue color. The color signal is captured using a smartphone and analyzed using color analysis software. A series of factors that affect the performance of the device are investigated, including the concentration of TMB, buffer pH, channel selection, and irradiation time. The analytical performance is evaluated, and the results show that the method has a wide detection range of 0.05–1.0 mmol L
−1 as well as a high selectivity and reproducibility. The proposed method is applied for assaying AA in beverage samples and a VC tablet, and the results correspond to those obtained using the UV method. The results of the recovery test of human serum indicate that the proposed method is feasible in biological samples. This device is easy to fabricate, and convenient to store and transport. It is a truly portable device which has great potential in the on-site assay. [ABSTRACT FROM AUTHOR]- Published
- 2023
- Full Text
- View/download PDF
14. On paper characterisation of droplet and evaporation study using impedance spectroscopy.
- Author
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Siva Prakasam, O. Kare, Basu, Akashlina, Chaudhury, Koel, and Das, Soumen
- Published
- 2024
- Full Text
- View/download PDF
15. Water-resistant, strong, degradable and recyclable rosin-grafted cellulose composite paper.
- Author
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Sun, Penghao, Wang, Siheng, Huang, Zhen, Zhang, Lei, Dong, Fuhao, Xu, Xu, and Liu, He
- Subjects
CELLULOSE fibers ,CELLULOSE ,YOUNG'S modulus ,SUSTAINABLE development ,PLASTICS ,DRINKING straws - Abstract
Petroleum-based plastics are useful, but they pose a huge threat to the environment and human health. The development of sustainable cellulose paper materials with excellent mechanical and water-resistance properties to replace plastics is highly desirable but also challenging. Herein, we report a new kind of rosin-based modifier synthesized from fully hydrogenated rosin and (3-glycidoxypropyl)triethoxysilane, which can covalently bind with cellulose fiber networks to improve the hydrophobicity and weaken the capillarity of cellulose paper. The rosin-grafted cellulose composite paper shows a unique combination of water resistance (water absorption of 19.2% and 37.9% in 25 and 90 °C water, respectively) and high strength (tensile strength of 47.9 MPa and Young's modulus of 2.32 GPa). Moreover, the rosin-grafted cellulose composite paper can be completely degraded when buried in soil for up to 100 days, and it can be recycled when treated with an alkali. These impressive performances make rosin-grafted cellulose composite paper a promising eco-friendly alternative material to replace plastics in straws, boxes, cups, bags and so on. [ABSTRACT FROM AUTHOR]
- Published
- 2022
- Full Text
- View/download PDF
16. Rapid and inexpensive process to fabricate paper based microfluidic devices using a cut and heat plastic lamination process.
- Author
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Kumawat, Nityanand, Soman, Soja Saghar, Vijayavenkataraman, Sanjairaj, and Kumar, Sunil
- Subjects
LAMINATED plastics ,MICROFLUIDIC devices ,MICROFLUIDICS ,POROUS materials ,CHEMICAL industry ,FILTER paper ,CHEMICAL resistance - Abstract
Microfluidic paper-based analytical devices (microPADs) are emerging as simple-to-use, low-cost point-of-care testing platforms. Such devices are mostly fabricated at present by creating hydrophobic barriers using wax or photoresist patterning on porous paper sheets. Even though devices fabricated using these methods are used and tested with a wide variety of analytes, still they pose many serious practical limitations for low-cost automated mass fabrication for their widespread applicability. We present an affordable and simple two-step process – cut and heat (CH-microPADs) – for the selective fabrication of hydrophilic channels and reservoirs on a wide variety of porous media such as tissue/printing/filter paper and cloth types, such as cotton and polyester, by a lamination process. The technique presents many advantages as compared to existing commonly used methods. The devices possess excellent mechanical strength against bending, folding and twisting, making them virtually unbreakable. They are structurally flexible and show good chemical resistance to various solvents, acids and bases, presenting widespread applicability in areas such as clinical diagnostics, biological sensing applications, food processing, and the chemical industry. Fabricated paper media 96 well-plate CH-microPAD configurations were tested for cell culture applications using mice embryonic fibroblasts and detection of proteins and enzymes using ELISA. With a simple two-step process and minimal human intervention, the technique presents a promising step towards mass fabrication of inexpensive disposable diagnostic devices for both resource-limited and developed regions. [ABSTRACT FROM AUTHOR]
- Published
- 2022
- Full Text
- View/download PDF
17. Dual-response fluorescence sensing of H2PO4− and CO32− using AJP filter paper based on a pH-stable CdII-based luminescent metal–organic framework.
- Author
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Cao, Xiao-Qin, Li, Qiang, Yao, Shu-Li, Zhong, Li-qin, Cao, Lei, Chen, Yong-Qiang, and Liu, Sui-Jun
- Subjects
- *
METAL-organic frameworks , *FILTER paper , *FLUORESCENCE , *LIGHT emitting diodes , *DUAL fluorescence - Abstract
A new CdII-based luminescent metal–organic framework (LMOF) with the formula {[Cd(BIBT)(NDC)]·solvents}n (JXUST-32, BIBT = 4,7-bi(1H-imidazol-1-yl)benzo-[2,1,3]thiadiazole and H2NDC = 2,6-naphthalenedicarboxylic acid) was successfully synthesized by a solvothermal method. JXUST-32 shows a two-dimensional (4,4)-connected network and exhibits significant fluorescence red shift and slight enhancement for H2PO4− and CO32− sensing with detection limits of 0.11 and 0.12 μM, respectively. In addition, JXUST-32 has good thermal stability, chemical stability and recyclability. Significantly, JXUST-32 represents a fluorescence red-shift dual response MOF sensor for H2PO4− and CO32− detection and the analytes can be identified by the naked eye, aerosol jet printing filter paper, light-emitting diode beads and luminescent films. [ABSTRACT FROM AUTHOR]
- Published
- 2023
- Full Text
- View/download PDF
18. Paper card-like electrochemical platform as a smart point-of-care device for reagent-free glucose measurement in tears.
- Author
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Fiore, Luca, Sinha, Ankita, Seddaoui, Narjiss, di Biasio, Jessica, Ricci, Federico, Stojanovic, Goran M., and Arduini, Fabiana
- Subjects
SMART devices ,GLUCOSE ,ELECTRONIC paper ,POLYVINYL chloride ,DETECTION limit ,BLOOD sugar monitors - Abstract
This communication describes the development of polyvinyl chloride electrochemical system in which a paper layer loaded with reagents is inserted into the device, demonstrating a new concept of a paper card-like pad for a reagent-free and easy measurement of the target analyte in solution. This device detects glucose in artificial tears in the range of 0.2–2 mM with a detection limit of 50 μM by simply adding the artificial tears to the paper card-like pad. The novel configuration goes beyond the state of the art, widening the application range of paper in the design of smart analytical devices. [ABSTRACT FROM AUTHOR]
- Published
- 2023
- Full Text
- View/download PDF
19. PCB-C4D coupled with paper-based microfluidic sampling for the rapid detection of liquid conductivity.
- Author
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Yang, Mingpeng, Cao, Mingyi, Zhang, Zhixuan, and Wang, Chaofan
- Published
- 2024
- Full Text
- View/download PDF
20. A dual paper-based nucleic acid extraction method from blood in under ten minutes for point-of-care diagnostics.
- Author
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Malpartida-Cardenas, Kenny, Baum, Jake, Cunnington, Aubrey, Georgiou, Pantelis, and Rodriguez-Manzano, Jesus
- Subjects
NUCLEIC acid isolation methods ,POINT-of-care testing ,DRUG monitoring ,FILTER paper ,EUKARYOTIC cells - Abstract
Nucleic acid extraction (NAE) plays a crucial role for diagnostic testing procedures. For decades, dried blood spots (DBS) have been used for serology, drug monitoring, and molecular studies. However, extracting nucleic acids from DBS remains a significant challenge, especially when attempting to implement these applications to the point-of-care (POC). To address this issue, we have developed a paper-based NAE method using cellulose filter papers (DBSFP) that operates without the need for electricity (at room temperature). Our method allows for NAE in less than 7 min, and it involves grade 3 filter paper pre-treated with 8% (v/v) igepal surfactant, 1 min washing step with 1× PBS, and 5 min incubation at room temperature in 1× TE buffer. The performance of the methodology was assessed with loop-mediated isothermal amplification (LAMP), targeting the human reference gene beta-actin and the kelch 13 gene from P. falciparum. The developed method was evaluated against FTA cards and magnetic bead-based purification, using time-to-positive (min) for comparative analysis. Furthermore, we optimised our approach to take advantage of the dual functionality of the paper-based extraction, allowing for elution (eluted disk) as well as direct placement of the disk in the LAMP reaction (in situ disk). This flexibility extends to eukaryotic cells, bacterial cells, and viral particles. We successfully validated the method for RNA/DNA detection and demonstrated its compatibility with whole blood stored in anticoagulants. Additionally, we studied the compatibility of DBSFP with colorimetric and lateral flow detection, showcasing its potential for POC applications. Across various tested matrices, targets, and experimental conditions, our results were comparable to those obtained using gold standard methods, highlighting the versatility of our methodology. In summary, this manuscript presents a cost-effective solution for NAE from DBS, enabling molecular testing in virtually any POC setting. When combined with LAMP, our approach provides sample-to-result detection in under 35 minutes. [ABSTRACT FROM AUTHOR]
- Published
- 2023
- Full Text
- View/download PDF
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