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During the 1830s, the use of improved microscopic techniques together with new histological methods, including tissue fixation, allowed more precise data to be obtained concerning the structure of nerve tissue of animals as well as humans. The present article, based on the translations of original texts never before published, brings together for the first time the discoveries of famous scholars Gustav Valentin, Robert Remak, and Jan Evangelista Purkyně, who made their significant discoveries in the field of neuroscience almost simultaneously and shows how their findings affected each other. In addition, this article also contains digitally remastered and reconstructed figures published in the original works of Valentin, Remak, and Purkyně and they are displayed for the first time in high quality. Although the fundamental discoveries of these famous scholars did not imply the discovery of nerve cells as we know them today, they were certainly a very important basis for further research of many other eminent scholars during the second half of the nineteenth century.