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Start Over Author "Perussia, B" Publisher rockefeller university press
27 results on '"Perussia, B"'

27. Natural killer (NK) cell-mediated cytotoxicity: differential use of TRAIL and Fas ligand by immature and mature primary human NK cells.

Author

Zamai L, Ahmad M, Bennett IM, Azzoni L, Alnemri ES, and Perussia B

Subjects
Antibodies, Monoclonal pharmacology, Antigens, CD analysis, Apoptosis drug effects, Apoptosis Regulatory Proteins, Calcium metabolism, Cell Degranulation, Cell Differentiation, Cells, Cultured, Fas Ligand Protein, Humans, Interleukins pharmacology, Killer Cells, Natural cytology, Killer Cells, Natural drug effects, Membrane Glycoproteins genetics, RNA, Messenger analysis, Receptors, Tumor Necrosis Factor metabolism, Recombinant Fusion Proteins pharmacology, TNF-Related Apoptosis-Inducing Ligand, Tumor Cells, Cultured, Tumor Necrosis Factor-alpha genetics, Tumor Necrosis Factor-alpha immunology, fas Receptor physiology, Cytotoxicity, Immunologic drug effects, Killer Cells, Natural immunology, Membrane Glycoproteins physiology, Tumor Necrosis Factor-alpha physiology
Abstract

Mature natural killer (NK) cells use Ca2+-dependent granule exocytosis and release of cytotoxic proteins, Fas ligand (FasL), and membrane-bound or secreted cytokines (tumor necrosis factor [TNF]-alpha) to induce target cell death. Fas belongs to the TNF receptor family of molecules, containing a conserved intracytoplasmic "death domain" that indirectly activates the caspase enzymatic cascade and ultimately apoptotic mechanisms in numerous cell types. Two additional members of this family, DR4 and DR5, transduce apoptotic signals upon binding soluble TNF-related apoptosis-inducing ligand (TRAIL) that, like FasL, belongs to the growing TNF family of molecules. Here, we report that TRAIL produced or expressed by different populations of primary human NK cells is functional, and represents a marker of differentiation or activation of these, and possibly other, cytotoxic leukocytes. During differentiation NK cells, sequentially and differentially, use distinct members of the TNF family or granule exocytosis to mediate target cell death. Phenotypically immature CD161(+)/CD56(-) NK cells mediate TRAIL-dependent but not FasL- or granule release-dependent cytotoxicity, whereas mature CD56(+) NK cells mediate the latter two.

Published
1998
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