1. Positive selection of the peripheral B cell repertoire in gut-associated lymphoid tissues
- Author
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Dennis K. Lanning, Paul J. Jasper, Periannan Sethupathi, Katherine L. Knight, Malathy Shanmugam, and Ki Jong Rhee
- Subjects
Models, Molecular ,DNA, Complementary ,Lymphoid Tissue ,Gut-associated lymphoid tissue ,Molecular Sequence Data ,Immunology ,Immunoglobulin Variable Region ,Fluorescent Antibody Technique ,Article ,Antibody Repertoire ,medicine ,Superantigen ,Animals ,Immunology and Allergy ,Amino Acid Sequence ,Cloning, Molecular ,Immunoglobulin Allotypes ,health care economics and organizations ,B cell ,B-Lymphocytes ,Binding Sites ,Superantigens ,Base Sequence ,Genes, Immunoglobulin ,biology ,Sequence Analysis, DNA ,Flow Cytometry ,Immunoglobulin Class Switching ,Molecular biology ,Allotype ,medicine.anatomical_structure ,Immunoglobulin M ,Immunoglobulin class switching ,biology.protein ,Calcium ,Rabbits ,Antibody - Abstract
Gut-associated lymphoid tissues (GALTs) interact with intestinal microflora to drive GALT development and diversify the primary antibody repertoire; however, the molecular mechanisms that link these events remain elusive. Alicia rabbits provide an excellent model to investigate the relationship between GALT, intestinal microflora, and modulation of the antibody repertoire. Most B cells in neonatal Alicia rabbits express V(H)n allotype immunoglobulin (Ig)M. Within weeks, the number of V(H)n B cells decreases, whereas V(H)a allotype B cells increase in number and become predominant. We hypothesized that the repertoire shift from V(H)n to V(H)a B cells results from interactions between GALT and intestinal microflora. To test this hypothesis, we surgically removed organized GALT from newborn Alicia pups and ligated the appendix to sequester it from intestinal microflora. Flow cytometry and nucleotide sequence analyses revealed that the V(H)n to V(H)a repertoire shift did not occur, demonstrating the requirement for interactions between GALT and intestinal microflora in the selective expansion of V(H)a B cells. By comparing amino acid sequences of V(H)n and V(H)a Ig, we identified a putative V(H) ligand binding site for a bacterial or endogenous B cell superantigen. We propose that interaction of such a superantigen with V(H)a B cells results in their selective expansion.
- Published
- 2004