1. Cell landscape of larval and adult Xenopus laevis at single-cell resolution
- Author
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Yuan Liao, Lifeng Ma, Weigao E, Lei Yang, Xing Fang, Qile Guo, Fanwei Ruan, Jingjing Wang, Peijing Zhang, Zhongyi Sun, Haide Chen, Zhongliang Lin, Xueyi Wang, Xinru Wang, Huiyu Sun, Ming Chen, Yincong Zhou, Wanhua Shen, Xiaoping Han, and Guoji Guo
- Abstract
Using Microwell-seq, we analyzed more than 500,000 single cells covering all major Xenopus tissues to construct the first Xenopus cell landscape to date. Common cell lineage-specific transcription factors have been identified in vertebrates, including fish, amphibians and mammals. A comparison of larval and adult erythrocytes revealed larval and adult-specific hemoglobin subtypes and a cluster of erythrocytes containing both larval and adult hemoglobin, mainly at NF59. In addition, cell lineages originating from all three layers exhibited both antigen processing and presentation during metamorphosis, indicating a common regulatory mechanism in metamorphosis. Our study provides a comprehensive resource for research on Xenopus developmental biology.
- Published
- 2022