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The Farm animal Genotype-Tissue Expression (FarmGTEx, https://www.farmgtex.org/) project has been established to develop a comprehensive public resource of genetic regulatory variants in domestic animal species, which is essential for linking genetic polymorphisms to variation in phenotypes, helping fundamental biology discovery and exploitation in animal breeding and human biomedicine. Here we present results from the pilot phase of PigGTEx (http://piggtex.farmgtex.org/), where we processed 9,530 RNA-sequencing and 1,602 whole-genome sequencing samples from pigs. We build a pig genotype imputation panel, characterize the transcriptional landscape across over 100 tissues, and associate millions of genetic variants with five types of transcriptomic phenotypes in 34 tissues. We study interactions between genotype and breed/cell type, evaluate tissue specificity of regulatory effects, and elucidate the molecular mechanisms of their action using multi-omics data. Leveraging this resource, we decipher regulatory mechanisms underlying about 80% of the genetic associations for 207 pig complex phenotypes, and demonstrate the similarity of pigs to humans in gene expression and the genetic regulation behind complex phenotypes, corroborating the importance of pigs as a human biomedical model.

Background To evaluate the efficacy and safety of percutaneous kyphoplasty (PKP) for thoracolumbar osteoporotic vertebral compression fracture (OVCF) with kyphosis via unilateral versus bilateral approach. Methods All patients suffered OVCF with kyphosis were retrospectively reviewed. Of those, performed unilateral PKP or underwent bilateral PKP with random. The clinical and radiological data such as the correction of deformity, sagittal profle and record of the perioperative morbidity of the patients were analyzed. Results All patients (76 ± 3.6 years) were enrolled in this investigation, including 41 in the unilateral and 45 in the bilateral group. No significant difference in general data was detected between the two groups (p > 0.05). Howere, the operation time and cement amount 28.2 ± 3.4 min, 3.8 ± 0.6 ml in the unilateral group, while 50.1 ± 4.6 min, 5.4 ± 0.5 ml in the bilateral group, respectively (P 0.05). Conclusions Both bilateral and unilateral PKP are relatively safe and provide effective treatment for patients with painful thoracolumbar osteoporotic vertebral compression fracture with mild kyphotic deformity. However, unilateral PKP need less operation time and volume of cement.

Objective: To evaluate the efficacy and safety of percutaneous kyphoplasty (PKP) for thoracolumbar osteoporotic vertebral compression fracture (OVCF) with kyphosis via unilateral versus bilateral approach.Methods: All patients suffered OVCF with kyphosis were retrospectively reviewed. Of those, performed unilateral PKP or underwent bilateral PKP with random. The clinical and radiological data such as the correction of deformity, sagittal profle and record of the perioperative morbidity of the patients were analyzed.Results: All patients (76±3.6 years) were enrolled in this investigation, including 47 in the unilateral and 39 in the bilateral group. No significant difference in general data was detected between the two groups (p >0.05). Howere, the operation time and cement amount 28.2±3.4 min, 3.8±0.6 ml in the unilateral group, while 50.1±4.6 min, 5.4±0.5 ml in the bilateral group, respectively (P 0.05).Conclusion: Both bilateral and unilateral PKP are relatively safe and provide effective treatment for patients with painful thoracolumbar osteoporotic vertebral compression fracture with mild kyphotic deformity. However, unilateral PKP need less operation time and volume of cement.

Purpose: To evaluate the therapeutic effects of short-segment posterior fixation with monoaxial pedicle screw or polyaxial pedicle screw via injured vertebra on thoracolumbar fracture.Methods: All patients who underwent short-segment posterior fixation with monoaxial pedicle screws or polyaxial pedicle screws in the injured vertebra of a thoracolumbar fracture (T12-L2). The clinical and radiological data such as the correction of deformity, sagittal profle and record of the perioperative morbidity of the patients were analyzed.Results: There were 63 patients (21 males and 42 females) with an average age of 44.7 years and were categorised into two groups: monoaxial pedicle screws group (group A) and polyaxial pedicle screws group (group B). There were no significant differences in age, gender, fracture site, TLISS Score, ASIA Score, AO Classification, hospital stay, Injury-to-operation intereval, and associated injury between the two groups (P>0.05). Howere, compared with group B, the injury vertebral endplate centre ratio significantly increased postoperatively and at the final follow-up (PConclusion: Short-segment posterior fixation with monoaxial or polyaxial pedicle screws via the fracture level for thoracolumbar fracture can achieve kyphosis correction, reduce sagittal alignment correction failure, and maintain anterior vertebral height. The insertion of monoaxial pedicle screws at the fracture level after thoracolumbar vertebral fracture has a flick up ffect on the central vertebral body of the injured vertebrae, which is beneficial to the recovery of the vertebral endplate.

Objective To evaluate the efficacy of percutaneous kyphoplasty (PKP) for thoracolumbar osteoporotic vertebral compression fractures (OVCFs) via unilateral versus bilateral approach. Methods All patients who underwent PKP surgery for OVCFs in our hospital between June 2016 and December 2018 were included in this study. The pedicles were divided into two groups according to the manner of vertebral body puncture, which were as follows: unilateral pedicle puncture group (unilateral group, n=47) and bilateral pedicle puncture group (bilateral group, n=39).The operative time, amount of cement perfusion, correction angle of kyphosis, pain score before and after surgery, and leakage rate of bone cement were evaluated in all patients. The average follow-up was 19 months (range 13-34 months). Results The average age of patients who met the inclusion criteria, but not the exclusion criteria, was 76 years, and a total of 121 vertebral bodies were studied. There were 7 cases of postoperative bone cement leakage (unilateral group, 14.9%) and 6 cases of postoperative bone cement leakage (bilateral groups, 15.4%). There were differences in operative time and amount of cement perfusion between the two groups were statistically significant (P0.05). Moreover, there was no significant difference in sex and bone cement leakage rate between the two groups by Pearson x 2 test (P>0.05). Conclusion Patients with OVCFs could obtain similar satisfactory clinical results via both unilateral and bilateral PKP approaches. However, the unilateral PKP approach is more advantageous as it has a shorter operative time, requires lesser cement volume, causes minimal trauma, is less costly, and results in lesser complications than the bilateral approach.

Purpose: To evaluate the use of short-segment posterior fixation with monoaxial and polyaxial pedicle screws in thoracolumbar fractures Methods: All patients who underwent short-segment posterior fixation with monoaxial pedicle screws or polyaxial pedicle screws in the injured vertebra of a thoracolumbar fracture (T12-L2) in our hospital between June 2012 and December 2018 were categorised into two groups: monoaxial pedicle screws group (group A) and polyaxial pedicle screws group (group B). We compared the Thoracolumbar Injury Severity Score (TLISS), American Spinal Injury Association (ASIA) score, the fracture level, Arbeitsgemeinschaft für Osteosynthesefragen (AO) classification, hospital stay, injury-to-operation interval, and associated injuries between the two groups. In addition, the prevertebral height ratio, the injured vertebra Cobb angle, and the injured vertebral endplate centre ratio were measured preoperatively, postoperatively, and at the final follow-up. Results: There were 63 patients (21 males and 42 females) with an average age of 44.7 years. Compared with group B, the injury vertebral endplate centre ratio significantly increased postoperatively and at the final follow-up (P