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Start Over Author "Jason C. Klima" Publisher research square platform llc
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1. Bacterial expression and protein purification of mini-fluorescence-activating proteins

Author

Cameron M. Chow, Jason C. Klima, Jiayi Dou, David Baker, Lauren Carter, and Anastassia A. Vorobieva

Subjects
Text mining, Biochemistry, business.industry, Chemistry, Protein purification, business, Fluorescence
Abstract

Bacterial expression and purification of de novo designed mini-fluorescence-activating proteins (mFAPs) are accomplished by genetically fusing mFAP variants to an N-terminal 6xHis tag and optionally to a Tobacco Etch Virus (TEV) protease epitope. The protocol can take less than 24 hours to complete, and allows for downstream in vitro characterization of photophysical properties of mFAPs by equilibration with exogenous fluorogenic compounds.

Published
2021
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