Search

Today, nanoparticles have promised a variety of applications in conjugation with biomolecules. Selenium nanoparticles (SeNPs) possess promising potency in a wide range of biomedicine applications, and amyloid fibrils with well-known physicochemical stability and ability to mimic the extracellular matrix (ECM) have been highlighted with possible use in the design of high-yield materials. To exploit the dual properties of nano and bio components in biomedical applications, this research has focused on the development of a nanohybrid system through assembling SeNPs on lysozyme amyloid fibrils. Spectroscopic and microscopic techniques including UV-vis, Far-UV Circular Dichroism (CD) spectropolarimetry, Thioflavin T (ThT) assay, and Transmission Electron microscopy were used to characterize the formation of lysozyme amyloid fibrils, selenium nanoparticles, and their assemblies on the biotemplate. Antibacterial activity of the individual components, as well as the nanohybrid system, was determined against Escherichia coli and Staphylococcus aureus using the minimum inhibitory concentration method. The nanohybrid system showed enhanced antibacterial activity upon the interaction of SeNPs with amyloid fibrils. Moreover, a study on the electronic behavior of the hybrid nanoscaffold by Electrochemical impedance spectroscopy (EIS) showed improved conductivity. The results suggest that the combination of nanoparticles with amyloid fibrils paves the way for the development of versatile nanoscaffolds with improved properties in regenerative medicine.