Because some laboratory personnel in colleges and universities lack safety consciousness, the laboratory safety management system is not sound and safety education work does not reach the designated position，causes sudden accidents have occurred frequently. These accidents seriously affects the normal operation of the teaching, and scientific research and development. Through the analysis of the relationship of laboratory safety management and psychological intervention, the paper aimed at the safety accident prevention and management of laboratory, establishing presented safety accident management of psychological intervention mechanism. From the "four square", psychological regulation network that are organization adjustment, leadership adjustment, peer adjustment and self adjustment of psychological regulation network of "four square", we using used the correct psychological intervention method, and through the establishment ofed a stable psychological security network, to avoid the mistakes resulted bystrengthening human behavior and reasonable use in the experimental processes, of the mistakes of human behavior control and reasonable use of We also applied positive reinforcement or psychological crisis intervention methods such as laboratory safety before the accident, to realize accident prevention and intervention, of the control and guidance in, accidents, psychological later adjustment and improvement after accidents, to These measures ensured that the human factor played a right role in the laboratory safety accidents management play a right role. [ABSTRACT FROM AUTHOR]