1. Acute administration of levetiracetam in tonic pain model modulates gene expression of 5HT 1A and 5HT 7 receptors in the thalamus of rats (Rattus norvergicus).
- Author
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Cortes-Altamirano JL, Reyes-Long S, Bonilla-Jaime H, Clavijo-Cornejo D, Vargas J, Bandala C, and Alfaro-Rodriguez A
- Subjects
- Animals, Brain metabolism, Dose-Response Relationship, Drug, Gene Expression drug effects, Gene Expression genetics, Gene Expression Regulation drug effects, Gene Expression Regulation genetics, Levetiracetam metabolism, Male, Pain drug therapy, Pain genetics, Pain Measurement methods, Rats, Rats, Wistar, Receptor, Serotonin, 5-HT1A metabolism, Receptors, Serotonin metabolism, Receptors, Serotonin physiology, Serotonin Plasma Membrane Transport Proteins metabolism, Thalamus metabolism, Levetiracetam pharmacology, Receptor, Serotonin, 5-HT1A genetics, Receptors, Serotonin genetics
- Abstract
The nociceptive effect of Levetiracetam (LEV) on the expression of 5-HT
1A and 5-HT7 receptors found in the thalamus was evaluated. Thirty-six male rats (Wistar) were randomized into six groups: in the Control group without treatment; LEV50 group LEV was administered in a single dose of 50 mg/kg i.g.; in the LEV300 group LEV dose of 300 mg/kg i.g.; in the FORMALIN group the formalin test was performed; in the LEV50/FORMALIN group LEV dose of 50 mg/kg i.g and the formalin test was performed; in the LEV300/FORMALIN group LEV dose of 300 mg/kg i.g and the formalin test was performed, subsequently the thalamus was dissected in all groups. In the formalin tests LEV exhibited an antinociceptive effect in the LEV300/FORMALIN group (p < 0.05) and a pronociceptive effect in the LEV50/FORMALIN group (p < 0.001). The results obtained by Real-time PCR confirmed the expression of the 5-HT1A and 5-HT7 receptors in the thalamus, 5-HT1A receptors increased significantly in the FORMALIN group and the LEV300/FORMALIN group (p < 0.05). 5-HT7 receptors are only over expressed at a dose of 300 mg/Kg of LEV with formalin (p < 0.05). This suggests that LEV modulates the sensation of pain by controlling the expression of 5-HT1A and 5-HT7 in a tonic pain model, and that changes in the expression of 5-HT1A and 5-HT7 receptors are associated with the sensation of pain, furthermore its possibility to be used in clinical treatments for pain.- Published
- 2020
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