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Objective: To analyze the expression and diagnostic value of plasma irisin and growth differentiation factor-8 in elderly patients with sarcopenia. Methods: A total of 620 elderly patients who received physical examination in our hospital from April 2020 to April 2022 were selected as the study subjects, including 238 patients with sarcopenia as the observation group and 382 patients without sarcopenia as the control group. The expression levels of plasma irisin and growth differentiation factor-8 in the two groups were detected to observe the differences of plasma irisin and growth differentiation factor-8 in elderly patients with different degrees of sarcopenia. Pearson correlation was used to analyze the relationship between plasma irisin, growth differentiation factor-8 and total skeletal muscle mass, limb skeletal muscle mass, relative skeletal muscle index,grip strength and walking speed, and the diagnostic efficacy of plasma irisin and growth differentiation factor-8 in senile sarcopenia was evaluated by area under receiver operating characteristic curve (AUC). Results: The expression level of plasma irisin in the observation group was lower than that in the control group, and the expression level of growth differentiation factor-8 was higher than that in the control group (P<0.05). The expression level of plasma irisin in patients with pre-sarcopenia, sarcopenia and severe sarcopenia was decreased successively, and the expression level of growth differentiation factor-8 was increased successively (P<0.05). Pearson correlation analysis showed that body skeletal muscle mass, limb skeletal muscle mass, relative skeletal muscle index, grip strength and walking speed were positively correlated with irisin in senile sarcopenia patients (P<0.05), and negatively correlated with growth differentiation factor-8 (P<0.05). ROC curve analysis showed that the sensitivity, specificity and AUC of plasma irisin and growth differentiation factor-8 for the diagnosis of senility sarcopenia were 78.49 %, 90.67 % and 0.914. Conclusion: The expression of plasma irisin and growth differentiation factor-8 in senile patients with sarcopenia is significantly down-regulated, which are closely related to the severity of the disease, the combined diagnosis of this disease is effective and worthy of clinical attention. [ABSTRACT FROM AUTHOR]

Objective: To investigate the effect of TRB3 gene knockout on skeletal muscle atrophy and fibrosis in diabetic mice.Methods: Thirty TRB3 knockout(TRB3-/-) mice and 30 C57/BL6J mice were randomly divided into 4 groups, including TRB3 knockout diabetes model group(group A), normal TRB3 knockout control group(group B), normal control group(group C) and diabetes model group(group D). Normal control group mice were fed standard rat feed, and diabetic mice were injected intraperitoneally with low dose of streptozotocin to establish diabetic mice model. The forelimb grip strength test, suspension grid test and muscle function test were performed. Meanwhile, the levels of bone microstructure, anti-type I collagen and anti-type III collagen, atrophy gene MuRF1 and atrophy gene atrophy 1 were determined. Results: The hand grip strength of group A, B and D was significantly lower than that of group C, and the hand grip strength of group D was the lowest(P＜0.05). The inverted hanging time of mice in groups A, B and D was significantly lower than that in group C, and the inverted hanging time of mice in group D was the shortest(P＜0.05). CSA value of muscle function in groups A, B and D was significantly lower than that in group C, and CSA value of muscle function in group D was the lowest(P＜0.05). The bone microstructure indexes BMD, BV/TV, Tb.N and Tb.Th in groups A, B and D were significantly lower than those in group C, and the lowest values were in group D(P＜0.05). The values of Tb.Sp and SMI in group C were significantly higher than those in group C, and the values in group D were the highest(P＜0.05). The levels of anti-type I collagen and anti-type III collagen in groups A, B and D were significantly higher than those in group C, and the levels of anti-type I collagen and anti-type III collagen in group D were the highest(P＜0.05).The levels of atrophy gene MuRF1 and Atrogin-1 in groups A, B and D were significantly higher than those in group C, and the levels of Mu RF1 and Atrogin-1 in group D were the highest(P＜0.05). Conclusion: TRB3 gene knockout significantly reduced skeletal muscle atrophy and fibrosis and increased bone strength in diabetic mice. [ABSTRACT FROM AUTHOR]

Objective: To investigate the effects of exosome miR-338 on bone metabolism, trabecular microstructure and bone biodynamics in osteoporosis rats. Methods: Bone marrow mesenchymal stem cells (BMSCs) were isolated from healthy adult SPF SD male rats. A rat model of osteoporosis was established by bilateral ovariectomy. The expression level of miR-338 was detected by qRT-PCR. Bone mineral density, trabecular microstructure and bone biomechanics were measured. Results: Compared with blank control group, the expression level of miR-338 was higher in OP model group, OP+ ExoBMSCs, inhibition group and overexpression group (P<0.05). The expression level of miR-338 in inhibition group was lower than that in OP model group, OP+ ExoBMSCs and overexpression group (P-0.05). Compared with blank control group, the expression levels of OC, PINP and BALP in OP model group, OP+ ExoBMSCs, inhibition group and overexpression group were lower (P<0.05). The expression levels of OC, PINP and BALP in inhibition group were higher than those in OP model group, OP+ ExoBMSCs and overexpression group (P<0.05). Compared with blank control group, the levels of BV/TV, Th.N, Tb.Th and Conn. D in OP model group, OP+ ExoBMSCs, inhibition group and overexpression group were lower, Tb.Sp and SMI were higher (P<0.05). The levels of BV/TV, Th.N, Tb.Th and Conn.D in inhibition group were higher than those in OP model group, OP+ ExoBMSCs and overexpression group (P<0.05), Tb.Sp and SMI were lower (P<0.05). Compared with blank control group, the levels of BMD, maximum load, maximum stress, maximum displacement and stiffness in OP model group, OP+ ExoBMSCs, inhibition group and overexpression group were lower (P<0.05). The levels of BMD, maximum load, maximum stress, maximum displacement and stiffness in inhibition group were higher than those in OP model group, OP+ ExoBMSCs and overexpression group (P<0.05). Conclusion: BMSCS-derived miR-338 can affect bone metabolism, trabecular microstructure and bone biomechanical status in osteoporosis rats. [ABSTRACT FROM AUTHOR]

Copyright of Progress in Modern Biomedicine is the property of Publishing House of Progress in Modern Biomedicine and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Progress in Modern Biomedicine is the property of Publishing House of Progress in Modern Biomedicine and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Progress in Modern Biomedicine is the property of Publishing House of Progress in Modern Biomedicine and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Objective: To explore the clinical effect of hysteroscope in treatment of intrauterine adhesion(IUA) and analyze the risk factors for IUA. Methods: A total of 213 patients, who underwent abortion in University City Affiliated Hospital of Medical University Of Chongqing from June 2014 to June 2015, were selected and divided into adhesion group(n=103) and no adhesion group(n=110) according to the status of IUA, and the adhesion group underwent surgery under hysteroscope. The basic information and relevant disease history of the two groups were recorded. The risk factors of IUA and the curative effectof adhesion group were analyzed. Results: The single factor analysis showed that there were significant differences in the gravidity times, negative pressure of suck palace and time of suck palace between the two groups(P<0.05). Multiple factor analysis showed negative pressure of suck palace and time of suck palace were the risk factors for IUA(P<0.05). The total effective rates of mild, moderate and severe IUA patients were 100. 00%, 88. 24% and75. 00% respectively after operation under hysteroscopy, the differences were statistically significant(x2=13. 870, P=0.001). Conclusion:Negative pressure of suck palace and time of suck palace are the risk factors of IUA, and hysteroscope has a good effect in treatment of IUA, which is worthy of clinical application. [ABSTRACT FROM AUTHOR]