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Copyright of Progress in Modern Biomedicine is the property of Publishing House of Progress in Modern Biomedicine and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Objective: To purify recombinant lentils allergens Len c1 (rLen c 1) and identify the immune activity. Methods: By means of prokaryotic expression system rLen c 1 was produced , then the target protein with Strep Ⅱ tag was purified with the method of affinity chromatography. BALB/c mice were randomly divided into control group (injected by normal saline) and allergen sensitized group (injected by rLen c 1). The mice were immuned by intraperitoneal injection to set up BALB/c mice lentils allergic model, then the total IgE and allergen specific IgE were detected by indirect ELISA to identify the immune activity of recombinant lentil allergen. Results: Len c 1 protein expression was successfully induced by IPTG，which was expressed in the form of inclusion body; with dialysis renaturation and affinity chromatography,the highly purified renatured protein rLen c 1 was gained, and mice sensitization model was established successfully. Compared with the control group of mice, the TIgE and allergen specific IgE significantly increased in sensitized group of mice. Conclusions: Purified rLen c 1 allergen protein with immune activity was obtained to lay a foundation for the preparation of monoclonal antibodies, clinical diagnosis and immunotherapy. [ABSTRACT FROM AUTHOR]

Copyright of Progress in Modern Biomedicine is the property of Publishing House of Progress in Modern Biomedicine and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)