1. Circulating Tumor Cells Count and Morphological Features in Breast, Colorectal and Prostate Cancer
- Author
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Marco R. De Groot, Cornelis J. A. Punt, Sjoerd Ligthart, Frank A. W. Coumans, Leon W.M.M. Terstappen, Lieke H.J. Simkens, Gerhardt Attard, Johann S. de Bono, Jean-Yves Pierga, François-Clément Bidard, Medical Cell Biophysics, ACS - Amsterdam Cardiovascular Sciences, CCA -Cancer Center Amsterdam, Biomedical Engineering and Physics, Graduate School, and Oncology
- Subjects
Male ,Oncology ,Colorectal cancer ,Kaplan-Meier Estimate ,Pattern Recognition, Automated ,Benign tumor ,Prostate cancer ,Engineering ,Circulating tumor cell ,Prostate ,Image Processing, Computer-Assisted ,Pathology ,Aged, 80 and over ,Univariate analysis ,Multidisciplinary ,Applied Mathematics ,Middle Aged ,Neoplastic Cells, Circulating ,Prognosis ,medicine.anatomical_structure ,Medicine ,Colorectal Neoplasms ,Algorithms ,Cancer Screening ,Research Article ,Test Evaluation ,Adult ,medicine.medical_specialty ,Science ,Breast Neoplasms ,Bioengineering ,Medical Devices ,Breast cancer ,Diagnostic Medicine ,Internal medicine ,Cancer Detection and Diagnosis ,medicine ,Humans ,Aged ,business.industry ,Prostatic Neoplasms ,medicine.disease ,Blood Cell Count ,Computer Science ,Cancer biomarkers ,business ,Mathematics ,Biomarkers ,Follow-Up Studies ,General Pathology - Abstract
BackgroundPresence of circulating tumor cells (CTC) in patients with metastatic breast, colorectal and prostate cancer is indicative for poor prognosis. An automated CTC (aCTC) algorithm developed previously to eliminate the variability in manual counting of CTC (mCTC) was used to extract morphological features. Here we validated the aCTC algorithm on CTC images from prostate, breast and colorectal cancer patients and investigated the role of quantitative morphological parameters.MethodologyStored images of samples from patients with prostate, breast and colorectal cancer, healthy controls, benign breast and colorectal tumors were obtained using the CellSearch system. Images were analyzed for the presence of aCTC and their morphological parameters measured and correlated with survival.ResultsOverall survival hazard ratio was not significantly different for aCTC and mCTC. The number of CTC correlated strongest with survival, whereas CTC size, roundness and apoptosis features reached significance in univariate analysis, but not in multivariate analysis. One aCTC/7.5 ml of blood was found in 7 of 204 healthy controls and 9 of 694 benign tumors. In one patient with benign tumor 2 and another 9 aCTC were detected.Significance of the studyCTC can be identified and morphological features extracted by an algorithm on images stored by the CellSearch system and strongly correlate with clinical outcome in metastatic breast, colorectal and prostate cancer.
- Published
- 2013