7 results on '"F. Zerbini"'
Search Results
2. Candida albicans PPG1, a serine/threonine phosphatase, plays a vital role in central carbon metabolisms under filament-inducing conditions: A multi-omics approach.
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Mohammad Tahseen A L Bataineh, Nelson Cruz Soares, Mohammad Harb Semreen, Stefano Cacciatore, Nihar Ranjan Dash, Mohamad Hamad, Muath Khairi Mousa, Jasmin Shafarin Abdul Salam, Mutaz F Al Gharaibeh, Luiz F Zerbini, and Mawieh Hamad
- Subjects
Medicine ,Science - Abstract
Candida albicans is the leading cause of life-threatening bloodstream candidiasis, especially among immunocompromised patients. The reversible morphological transition from yeast to hyphal filaments in response to host environmental cues facilitates C. albicans tissue invasion, immune evasion, and dissemination. Hence, it is widely considered that filamentation represents one of the major virulence properties in C. albicans. We have previously characterized Ppg1, a PP2A-type protein phosphatase that controls filament extension and virulence in C. albicans. This study conducted RNA sequencing analysis of samples obtained from C. albicans wild type and ppg1Δ/Δ strains grown under filament-inducing conditions. Overall, ppg1Δ/Δ strain showed 1448 upregulated and 710 downregulated genes, representing approximately one-third of the entire annotated C. albicans genome. Transcriptomic analysis identified significant downregulation of well-characterized genes linked to filamentation and virulence, such as ALS3, HWP1, ECE1, and RBT1. Expression analysis showed that essential genes involved in C. albicans central carbon metabolisms, including GDH3, GPD1, GPD2, RHR2, INO1, AAH1, and MET14 were among the top upregulated genes. Subsequent metabolomics analysis of C. albicans ppg1Δ/Δ strain revealed a negative enrichment of metabolites with carboxylic acid substituents and a positive enrichment of metabolites with pyranose substituents. Altogether, Ppg1 in vitro analysis revealed a link between metabolites substituents and filament formation controlled by a phosphatase to regulate morphogenesis and virulence.
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- 2021
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3. Correction: Genomic Counter-Stress Changes Induced by the Relaxation Response.
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Jeffery A Dusek, Hasan H Otu, Ann L Wohlhueter, Manoj Bhasin, Luiz F Zerbini, Marie G Joseph, Herbert Benson, and Towia A Libermann
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Medicine ,Science - Abstract
[This corrects the article DOI: 10.1371/journal.pone.0002576.].
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- 2017
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4. Genomic counter-stress changes induced by the relaxation response
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Marie G. Joseph, Ann L. Wohlhueter, Hasan H. Otu, Jeffery A. Dusek, Luiz F. Zerbini, Towia A. Libermann, Manoj Bhasin, and Herbert Benson
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medicine.medical_specialty ,lcsh:Medicine ,Oxidative phosphorylation ,Disease ,Relaxation Therapy ,Biology ,medicine.disease_cause ,Bioinformatics ,03 medical and health sciences ,0302 clinical medicine ,Internal medicine ,Gene expression ,medicine ,Humans ,030212 general & internal medicine ,Genetics and Genomics/Genomics ,lcsh:Science ,Gene ,chemistry.chemical_classification ,Regulation of gene expression ,Reactive oxygen species ,Multidisciplinary ,Gene Expression Profiling ,lcsh:R ,Mental Health/Psychology ,3. Good health ,Endocrinology ,Gene Expression Regulation ,chemistry ,Case-Control Studies ,lcsh:Q ,Signal transduction ,Stress, Psychological ,030217 neurology & neurosurgery ,Oxidative stress ,Research Article - Abstract
Background: Mind-body practices that elicit the relaxation response (RR) have been used worldwide for millennia to prevent and treat disease. The RR is characterized by decreased oxygen consumption, increased exhaled nitric oxide, and reduced psychological distress. It is believed to be the counterpart of the stress response that exhibits a distinct pattern of physiology and transcriptional profile. We hypothesized that RR elicitation results in characteristic gene expression changes that can be used to measure physiological responses elicited by the RR in an unbiased fashion. Methods/Principal Findings: We assessed whole blood transcriptional profiles in 19 healthy, long-term practitioners of daily RR practice (group M), 19 healthy controls (group N1), and 20 N1 individuals who completed 8 weeks of RR training (group N2). 2209 genes were differentially expressed in group M relative to group N1 (p,0.05) and 1561 genes in group N2 compared to group N1 (p,0.05). Importantly, 433 (p,10210) of 2209 and 1561 differentially expressed genes were shared among long-term (M) and short-term practitioners (N2). Gene ontology and gene set enrichment analyses revealed significant alterations in cellular metabolism, oxidative phosphorylation, generation of reactive oxygen species and response to oxidative stress in long-term and short-term practitioners of daily RR practice that may counteract cellular damage related to chronic psychological stress. A significant number of genes and pathways were confirmed in an independent validation set containing 5 N1 controls, 5 N2 short-term and 6 M long-term practitioners. Conclusions/Significance: This study provides the first compelling evidence that the RR elicits specific gene expression changes in short-term and long-term practitioners. Our results suggest consistent and constitutive changes in gene expression resulting from RR may relate to long term physiological effects. Our study may stimulate new investigations into applying transcriptional profiling for accurately measuring RR and stress related responses in multiple disease settings.
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- 2008
5. Differential Expression and Enzymatic Activity of DPPIV/CD26 Affects Migration Ability of Cervical Carcinoma Cells
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Aline Beckenkamp, Alessandra Nejar Bruno, Márcia Rosângela Wink, Danielle Bertodo Santana, Jéssica Nascimento, Diogo André Pilger, Juliano D. Paccez, Julia Biz Willig, Andréia Buffon, and Luiz F. Zerbini
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Keratinocytes ,Dipeptidyl Peptidase 4 ,lcsh:Medicine ,Uterine Cervical Neoplasms ,Biology ,Sitagliptin Phosphate ,HeLa ,Cell membrane ,Cell Movement ,Cell Line, Tumor ,Cell Adhesion ,medicine ,Humans ,lcsh:Science ,Cell adhesion ,Dipeptidyl-Peptidase IV Inhibitors ,Membrane Glycoproteins ,Multidisciplinary ,Cell growth ,lcsh:R ,Carcinoma ,Cell Membrane ,Farmácia ,Cell migration ,Proliferação celular ,Cancer cervico-uterino ,biology.organism_classification ,Molecular biology ,HaCaT ,medicine.anatomical_structure ,Cell culture ,lcsh:Q ,Female ,Dipeptidil peptidase 4 ,Research Article ,HeLa Cells - Abstract
Dipeptidyl peptidase IV (DPPIV/CD26) is a transmembrane glycoprotein that inactivates or degrades some bioactive peptides and chemokines. For this reason, it regulates cell proliferation, migration and adhesion, showing its role in cancer processes. This enzyme is found mainly anchored onto the cell membrane, although it also has a soluble form, an enzymatically active isoform. In the present study, we investigated DPPIV/CD26 activity and expression in cervical cancer cell lines (SiHa, HeLa and C33A) and non-tumorigenic HaCaT cells. The effect of the DPPIV/CD26 inhibitor (sitagliptin phosphate) on cell migration and adhesion was also evaluated. Cervical cancer cells and keratinocytes exhibited DPPIV/CD26 enzymatic activity both membrane-bound and in soluble form. DPPIV/CD26 expression was observed in HaCaT, SiHa and C33A, while in HeLa cells it was almost undetectable. We observed higher migratory capacity of HeLa, when compared to SiHa. But in the presence of sitagliptin SiHa showed an increase in migration, indicating that, at least in part, cell migration is regulated by DPPIV/CD26 activity. Furthermore, in the presence of sitagliptin phosphate, SiHa and HeLa cells exhibited a significant reduction in adhesion. However this mechanism seems to be mediated independent of DPPIV/CD26. This study demonstrates, for the first time, the activity and expression of DPPIV/CD26 in cervical cancer cells and the effect of sitagliptin phosphate on cell migration and adhesion.
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- 2015
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6. Combinatorial effect of non-steroidal anti-inflammatory drugs and NF-κB inhibitors in ovarian cancer therapy.
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Luiz F Zerbini, Rodrigo E Tamura, Ricardo G Correa, Akos Czibere, Jason Cordeiro, Manoj Bhasin, Fernando M Simabuco, Yihong Wang, Xuesong Gu, Linglin Li, Devanand Sarkar, Jin-Rong Zhou, Paul B Fisher, and Towia A Libermann
- Subjects
Medicine ,Science - Abstract
Several epidemiological studies have correlated the use of non-steroidal anti-inflammatory drugs (NSAID) with reduced risk of ovarian cancer, the most lethal gynecological cancer, diagnosed usually in late stages of the disease. We have previously established that the pro-apoptotic cytokine melanoma differentiation associated gene-7/Interleukin-24 (mda-7/IL-24) is a crucial mediator of NSAID-induced apoptosis in prostate, breast, renal and stomach cancer cells. In this report we evaluated various structurally different NSAIDs for their efficacies to induce apoptosis and mda-7/IL-24 expression in ovarian cancer cells. While several NSAIDs induced apoptosis, Sulindac Sulfide and Diclofenac most potently induced apoptosis and reduced tumor growth. A combination of these agents results in a synergistic effect. Furthermore, mda-7/IL-24 induction by NSAIDs is essential for programmed cell death, since inhibition of mda-7/IL-24 by small interfering RNA abrogates apoptosis. mda-7/IL-24 activation leads to upregulation of growth arrest and DNA damage inducible (GADD) 45 α and γ and JNK activation. The NF-κB family of transcription factors has been implicated in ovarian cancer development. We previously established NF-κB/IκB signaling as an essential step for cell survival in cancer cells and hypothesized that targeting NF-κB could potentiate NSAID-mediated apoptosis induction in ovarian cancer cells. Indeed, combining NSAID treatment with NF-κB inhibitors led to enhanced apoptosis induction. Our results indicate that inhibition of NF-κB in combination with activation of mda-7/IL-24 expression may lead to a new combinatorial therapy for ovarian cancer.
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- 2011
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7. Genomic counter-stress changes induced by the relaxation response.
- Author
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Jeffery A Dusek, Hasan H Otu, Ann L Wohlhueter, Manoj Bhasin, Luiz F Zerbini, Marie G Joseph, Herbert Benson, and Towia A Libermann
- Subjects
Medicine ,Science - Abstract
Mind-body practices that elicit the relaxation response (RR) have been used worldwide for millennia to prevent and treat disease. The RR is characterized by decreased oxygen consumption, increased exhaled nitric oxide, and reduced psychological distress. It is believed to be the counterpart of the stress response that exhibits a distinct pattern of physiology and transcriptional profile. We hypothesized that RR elicitation results in characteristic gene expression changes that can be used to measure physiological responses elicited by the RR in an unbiased fashion.We assessed whole blood transcriptional profiles in 19 healthy, long-term practitioners of daily RR practice (group M), 19 healthy controls (group N(1)), and 20 N(1) individuals who completed 8 weeks of RR training (group N(2)). 2209 genes were differentially expressed in group M relative to group N(1) (p
- Published
- 2008
- Full Text
- View/download PDF
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