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Your search keyword '"Adrivanio Baranoski"' showing total 2 results
Start Over Author "Adrivanio Baranoski" Publisher public library of science (plos)
2 results on '"Adrivanio Baranoski"'

1. In vitro evaluation of the protective effects of plant extracts against amyloid-beta peptide-induced toxicity in human neuroblastoma SH-SY5Y cells.

Author

Ana Luiza Sereia, Marcelo Tempesta de Oliveira, Adrivanio Baranoski, Leila Larisa Medeiros Marques, Fabianne Martins Ribeiro, Raquel Garcia Isolani, Daniela Cristina de Medeiros, Danielly Chierrito, Danielle Lazarin-Bidóia, Acácio Antonio Ferreira Zielinski, Cláudio Roberto Novello, Celso Vataru Nakamura, Mário Sérgio Mantovani, and João Carlos Palazzo de Mello

Subjects
Medicine, Science
Abstract

Alzheimer's disease (AD) is the most common form of dementia and has no cure. Therapeutic strategies focusing on the reduction of oxidative stress, modulation of amyloid-beta (Aβ) toxicity and inhibition of tau protein hyperphosphorylation are warranted to avoid the development and progression of AD. The aim of this study was to screen the crude extracts (CEs) and ethyl-acetate fractions (EAFs) of Guazuma ulmifolia, Limonium brasiliense, Paullinia cupana, Poincianella pluviosa, Stryphnodendron adstringens and Trichilia catigua using preliminary in vitro bioassays (acetylcholinesterase inhibition, antioxidant activity and total polyphenol content) to select extracts/fractions and assess their protective effects against Aβ25-35 toxicity in SH-SY5Y cells. The effect of the EAF of S. adstringens on mitochondrial membrane potential, lipid peroxidation, superoxide production and mRNA expression of 10 genes related to AD was also evaluated and the electropherogram fingerprints of EAFs were established by capillary electrophoresis. Chemometric tools were used to correlate the in vitro activities of the samples with their potential to be evaluated against AD and to divide extracts/fractions into four clusters. Pretreatment with the EAFs grouped in cluster 1 (S. adstringens, P. pluviosa and L. brasiliense) protected SH-SY5Y cells from Aβ25-35-induced toxicity. The EAF of S. adstringens at 15.62 μg/mL was able completely to inhibit the mitochondrial depolarization (69%), superoxide production (49%) and Aβ25-35-induced lipid peroxidation (35%). With respect to mRNA expression, the EAF of S. adstringens also prevented the MAPT mRNA overexpression (expression ratio of 2.387x) induced by Aβ25-35, which may be related to tau protein hyperphosphorylation. This is the first time that the neuroprotective effects of these fractions have been demonstrated and that the electropherogram fingerprints for the EAFs of G. ulmifolia, L. brasiliense, P. cupana, P. pluviosa and S. adstringens have been established. The study expands knowledge of the in vitro protective effects and quality control of the evaluated fractions.

Published
2019
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2. In vitro evaluation of the protective effects of plant extracts against amyloid-beta peptide-induced toxicity in human neuroblastoma SH-SY5Y cells

Author

Leila Larisa Medeiros Marques, Marcelo T. Oliveira, Cláudio Roberto Novello, Mário Sérgio Mantovani, Ana Luiza Sereia, Acácio Antonio Ferreira Zielinski, Danielle Lazarin-Bidóia, João Carlos Palazzo de Mello, Fabianne Ribeiro, Daniela Cristina de Medeiros, Raquel Garcia Isolani, Danielly Chierrito, Celso Vataru Nakamura, and Adrivanio Baranoski

Subjects
Antioxidant, Physiology, medicine.medical_treatment, Pharmacology, medicine.disease_cause, Alzheimer's Disease, Toxicology, Pathology and Laboratory Medicine, Biochemistry, Antioxidants, Capillary Electrophoresis, Lipid peroxidation, chemistry.chemical_compound, Neuroblastoma, 0302 clinical medicine, Medicine and Health Sciences, Energy-Producing Organelles, Membrane Potential, Mitochondrial, 0303 health sciences, Multidisciplinary, biology, Chemistry, Neurodegenerative Diseases, Tau Protein, Lipids, Mitochondria, Electrophysiology, Neuroprotective Agents, Neurology, Toxicity, Acetylcholinesterase, Medicine, Cellular Structures and Organelles, Microtubule-Associated Proteins, Research Article, Amyloid beta, Science, Bioenergetics, Research and Analysis Methods, Membrane Potential, 03 medical and health sciences, Electrophoretic Techniques, food, Cell Line, Tumor, Mental Health and Psychiatry, medicine, Paullinia cupana, Humans, RNA, Messenger, 030304 developmental biology, Amyloid beta-Peptides, Plant Extracts, Biology and Life Sciences, Proteins, Polyphenols, Cell Biology, biology.organism_classification, food.food, Cytoskeletal Proteins, Gene Expression Regulation, Cytoprotection, biology.protein, Stryphnodendron adstringens, Dementia, Mitochondrial Membrane, Lipid Peroxidation, Cholinesterase Inhibitors, Trichilia catigua, Reactive Oxygen Species, 030217 neurology & neurosurgery, Oxidative stress
Abstract

Alzheimer’s disease (AD) is the most common form of dementia and has no cure. Therapeutic strategies focusing on the reduction of oxidative stress, modulation of amyloid-beta (Aβ) toxicity and inhibition of tau protein hyperphosphorylation are warranted to avoid the development and progression of AD. The aim of this study was to screen the crude extracts (CEs) and ethyl-acetate fractions (EAFs) of Guazuma ulmifolia, Limonium brasiliense, Paullinia cupana, Poincianella pluviosa, Stryphnodendron adstringens and Trichilia catigua using preliminary in vitro bioassays (acetylcholinesterase inhibition, antioxidant activity and total polyphenol content) to select extracts/fractions and assess their protective effects against Aβ25–35 toxicity in SH-SY5Y cells. The effect of the EAF of S. adstringens on mitochondrial membrane potential, lipid peroxidation, superoxide production and mRNA expression of 10 genes related to AD was also evaluated and the electropherogram fingerprints of EAFs were established by capillary electrophoresis. Chemometric tools were used to correlate the in vitro activities of the samples with their potential to be evaluated against AD and to divide extracts/fractions into four clusters. Pretreatment with the EAFs grouped in cluster 1 (S. adstringens, P. pluviosa and L. brasiliense) protected SH-SY5Y cells from Aβ25-35-induced toxicity. The EAF of S. adstringens at 15.62 μg/mL was able completely to inhibit the mitochondrial depolarization (69%), superoxide production (49%) and Aβ25-35-induced lipid peroxidation (35%). With respect to mRNA expression, the EAF of S. adstringens also prevented the MAPT mRNA overexpression (expression ratio of 2.387x) induced by Aβ25–35, which may be related to tau protein hyperphosphorylation. This is the first time that the neuroprotective effects of these fractions have been demonstrated and that the electropherogram fingerprints for the EAFs of G. ulmifolia, L. brasiliense, P. cupana, P. pluviosa and S. adstringens have been established. The study expands knowledge of the in vitro protective effects and quality control of the evaluated fractions.

Published
2019

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