1. In vitro mouse spermatogenesis with an organ culture method in chemically defined medium
- Author
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Yuta Asayama, Tatsuma Yao, Noriaki Arakawa, Hiroyuki Sanjo, Takeru Abe, Takuya Sato, Hiroyuki Yamanaka, Masahiro Yao, Hisashi Hirano, Mitsuru Komeya, Takehiko Ogawa, Kumiko Katagiri, Yoko Ino, and Akio Matsuhisa
- Subjects
0301 basic medicine ,Male ,Physiology ,Retinoic acid ,lcsh:Medicine ,Biochemistry ,chemistry.chemical_compound ,Follicle-stimulating hormone ,Mice ,Reproductive Physiology ,Animal Cells ,Spermatocytes ,Testis ,Medicine and Health Sciences ,Testosterone ,Cell Cycle and Cell Division ,Organ Cultures ,lcsh:Science ,Mice, Inbred ICR ,Multidisciplinary ,Chromosome Biology ,Organic Compounds ,Age Factors ,Serum Albumin, Bovine ,Lipids ,Spermatids ,Meiosis ,Chemistry ,medicine.anatomical_structure ,Cell Processes ,Physical Sciences ,Triiodothyronine ,Biological Cultures ,Cellular Types ,Luteinizing hormone ,Research Article ,Signal Transduction ,Mice, Transgenic ,Tretinoin ,Biology ,In Vitro Techniques ,Organ culture ,Research and Analysis Methods ,Andrology ,03 medical and health sciences ,Organ Culture Techniques ,Albumins ,medicine ,Animals ,Spermatogenesis ,Spermatid ,Ethanol ,lcsh:R ,Organic Chemistry ,Chemical Compounds ,Biology and Life Sciences ,Proteins ,Cell Biology ,Luteinizing Hormone ,Sperm ,Spermatogonia ,Culture Media ,Mice, Inbred C57BL ,Chemically defined medium ,030104 developmental biology ,Germ Cells ,chemistry ,Alcohols ,lcsh:Q ,Cattle ,Follicle Stimulating Hormone - Abstract
We previously reported the successful induction and completion of mouse spermatogenesis by culturing neonatal testis tissues. The culture medium consisted of α-minimum essential medium (α-MEM), supplemented with Knockout serum replacement (KSR) or AlbuMAX, neither of which were defined chemically. In this study, we formulated a chemically defined medium (CDM) that can induce mouse spermatogenesis under organ culture conditions. It was found that bovine serum albumin (BSA) purified through three different procedures had different effects on spermatogenesis. We also confirmed that retinoic acid (RA) played crucial roles in the onset of spermatogonial differentiation and meiotic initiation. The added lipids exhibited weak promoting effects on spermatogenesis. Lastly, luteinizing hormone (LH), follicle stimulating hormone (FSH), triiodothyronine (T3), and testosterone (T) combined together promoted spermatogenesis until round spermatid production. The CDM, however, was not able to produce elongated spermatids. It was also unable to induce spermatogenesis from the very early neonatal period, before 2 days postpartum, leaving certain factors necessary for spermatogenic induction in mice unidentified. Nonetheless, the present study provided important basic information on testis organ culture and spermatogenesis in vitro.
- Published
- 2018