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The cDNA expression libraries that produce correct proteins are essential in facilitating the identification of protein-protein interactions. The 59-untranslated regions (UTRs) that are present in the majority of mammalian and non-mammalian genes are predicted to alter the expression of correct proteins from cDNA libraries. We developed a novel cDNA expression library from which 59-UTRs were removed using a mixture of polymerase chain reaction primers that complement the Kozak sequences we refer to as an ''in-frame cDNA library.'' We used this library with the protein complementation assay to identify two novel binding partners for ras-related ADP-ribosylation factor-like 11 (ARL11), cellular retinoic acid binding protein 2 (CRABP2), and phosphoglycerate mutase 1 (PGAM1). Thus, the in-frame cDNA library without 59-UTRs we describe here increases the chance of correctly identifying protein interactions and will have wide applications in both mammalian and non-mammalian detection systems. [ABSTRACT FROM AUTHOR]