1. The Non-Classical MAP Kinase ERK3 Controls T Cell Activation.
- Author
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Marquis, Miriam, Boulet, Salix, Mathien, Simon, Rousseau, Justine, Thébault, Paméla, Daudelin, Jean-François, Rooney, Julie, Turgeon, Benjamin, Beauchamp, Claudine, Meloche, Sylvain, and Labrecque, Nathalie
- Subjects
MITOGEN-activated protein kinase phosphatases ,T cell receptors ,CYTOLOGY ,LABORATORY mice ,ANTIGENS ,PHOSPHORYLATION ,CD3 antigen - Abstract
The classical mitogen-activated protein kinases (MAPKs) ERK1 and ERK2 are activated upon stimulation of cells with a broad range of extracellular signals (including antigens) allowing cellular responses to occur. ERK3 is an atypical member of the MAPK family with highest homology to ERK1/2. Therefore, we evaluated the role of ERK3 in mature T cell response. Mouse resting T cells do not transcribe ERK3 but its expression is induced in both CD4
+ and CD8+ T cells following T cell receptor (TCR)-induced T cell activation. This induction of ERK3 expression in T lymphocytes requires activation of the classical MAPK ERK1 and ERK2. Moreover, ERK3 protein is phosphorylated and associates with MK5 in activated primary T cells. We show that ERK3-deficient T cells have a decreased proliferation rate and are impaired in cytokine secretion following in vitro stimulation with low dose of anti-CD3 antibodies. Our findings identify the atypical MAPK ERK3 as a new and important regulator of TCR-induced T cell activation. [ABSTRACT FROM AUTHOR]- Published
- 2014
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