1. Comparison on Virulence and Immunogenicity of Two Recombinant Vaccinia Vaccines, Tian Tan and Guang9 Strains, Expressing the HIV-1 Envelope Gene.
- Author
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Rong Zhu, Weijin Huang, Wenbo Wang, Qiang Liu, Jianhui Nie, Shufang Meng, Yongxin Yu, and Youchun Wang
- Subjects
AUTISM spectrum disorders ,MOTION perception (Vision) ,ROBUST control ,DECISION making ,PARIETAL lobe ,BRAIN - Abstract
Background: The vaccinia virus Guang9 strain (VG9), derived from the vaccinia virus Tian Tan strain (VTT) has been found to be less virulent than VTT. Methodology/Principal Findings: To investigate whether VG9 could be a potential replicating virus vector, the TK genes in VG9 and VTT were replaced with the HIV-1 envelope gene via homologous recombination, resulting in the recombinant viruses, VG9-E and VTT-E. The biology, virulence, humoral and cellular immunological responses of VG9-E and VTT-E were evaluated. Our results indicated no obvious difference in range of host cells and diffusion between two recombinant viruses. Neurovirulence for VG9-E in weanling and suckling mice, and skin virulence in rabbits, were lower than that of VTT-E. The humoral immune responses, including binding antibody and neutralizing antibody responses, induced by VG9-E were not significantly different from those for VTT-E whilst IFN- γ response which represented cellular immune response induced by VG9-E was significantly higher than that did by VTT-E. Conclusions/Significance: Our results indicated that VG9-E was less virulent, yet induced higher cellular immune response than VTT-E. Therefore, it could be an ideal replicating vaccinia vector for HIV vaccine research and development. [ABSTRACT FROM AUTHOR]
- Published
- 2012
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